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Assay Detail

CHEMBL5164634

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HCT-116 cells expressing wild type p53 and MDM2 assessed as cell growth inhibition incubated for 4 days by CCK-8 assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCT-116
Confidence 1 — Organism
Curated By Autocuration

Target

HCT-116 (CHEMBL394)
Type CELL-LINE
Organism Homo sapiens

Publication

Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.
Zhang S, Yan Z, Li Y, Gong Y, Lyu X, Lou J, Zhang D, Meng X, Zhao Y.
J Med Chem (2022) 65 : 6207 -6230
PubMed 35420431 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.54 7.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5179430 1 7.36
CHEMBL5181559 1 7.29
CHEMBL5196857 1 7.22
CHEMBL5205792 1 6.83
CHEMBL5176086 1 6.82
CHEMBL5188708 1 6.65
CHEMBL5169689 1 6.57
CHEMBL5199459 1 6.51
CHEMBL5173513 1 6.48
CHEMBL5197279 1 6.31
CHEMBL5200857 1 6.20
CHEMBL5195734 1 6.14
CHEMBL191334 NUTLIN-3 1 5.87
CHEMBL5190459 1 5.33

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5179430 IC50 = 43.3 nM 7.36
CHEMBL5181559 IC50 = 50.7 nM 7.29
CHEMBL5196857 IC50 = 60.1 nM 7.22
CHEMBL5205792 IC50 = 147.0 nM 6.83
CHEMBL5176086 IC50 = 153.0 nM 6.82
CHEMBL5188708 IC50 = 224.0 nM 6.65
CHEMBL5169689 IC50 = 269.0 nM 6.57
CHEMBL5199459 IC50 = 308.0 nM 6.51
CHEMBL5173513 IC50 = 328.0 nM 6.48
CHEMBL5197279 IC50 = 491.0 nM 6.31
CHEMBL5200857 IC50 = 631.0 nM 6.20
CHEMBL5195734 IC50 = 719.0 nM 6.14
CHEMBL191334 NUTLIN-3 IC50 = 1366.0 nM 5.87
CHEMBL5190459 IC50 = 4665.0 nM 5.33