Assay Detail
Functional
CHEMBL5209604
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of the Glucose Transporter (GLUT1, SLC2A1) as assessed by a FRET based flow cytometry assay using a genetically-encoded biosensor for measuring free glucose (FLII12Pglu-700uDelta6) in HEK293 JumpIN TRex SLC2A1 WT-OE cells (PubChem AID: 1794830)
4
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Solute carrier family 2, facilitated glucose transporter member 1 (CHEMBL2535) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Flow cytometry transport assay for SLC2A1 using HEK293 JumpIN TRex SLC2A1 WT-OE cells
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 6.01 | 6.62 |
| EC50 | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4448899 | BAY-876 | — | 1 | 6.62 |
| CHEMBL411729 | CYTOCHALASIN B | — | 1 | 5.39 |
| CHEMBL1222250 | DEXTROSE | 4.0 | 1 | - |
| CHEMBL3092944 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4448899 | BAY-876 | IC50 | = | 240.0 | nM | 6.62 |
| CHEMBL411729 | CYTOCHALASIN B | IC50 | = | 4100.0 | nM | 5.39 |
| CHEMBL1222250 | DEXTROSE | EC50 | = | 527000.0 | nM | - |
| CHEMBL3092944 | — | IC50 | > | 10000.0 | nM | - |