Assay Detail
Functional
CHEMBL5209627
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Substrate uptake by the Na(+)/Dicarboxylate Cotransporter 3 (NADC3, SLC13A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC13A3 cells (PubChem AID: 1794820)
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Na(+)/dicarboxylate cotransporter 3 (CHEMBL3712947) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Membrane potential based assay for SLC13A3 using HEK-293 SLC13A3 OE cells
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 2 | 4.27 | 4.27 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1686 | ALPHA-KETOGLUTARIC ACID | — | 1 | 4.27 |
| CHEMBL576 | SUCCINIC ACID | 3.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1686 | ALPHA-KETOGLUTARIC ACID | EC50 | = | 54000.0 | nM | 4.27 |
| CHEMBL576 | SUCCINIC ACID | EC50 | = | 107000.0 | nM | - |