Assay Detail
Binding
CHEMBL5229153
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of ssDNA with ATTGTCA (m6A) CAGCAGA-FAM from human FTO measured after 30 mins by fluorescence polarization assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Alpha-ketoglutarate-dependent dioxygenase FTO (CHEMBL2331065) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibition of AlkB Nucleic Acid Demethylases: Promising New Epigenetic Targets.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 4.35 | 4.68 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL418068 | RHEIN | — | 1 | 4.68 |
| CHEMBL5279213 | — | — | 1 | 4.19 |
| CHEMBL270415 | BROMAMINIC ACID | — | 1 | 4.17 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL418068 | RHEIN | IC50 | = | 21000.0 | nM | 4.68 |
| CHEMBL5279213 | — | IC50 | = | 64000.0 | nM | 4.19 |
| CHEMBL270415 | BROMAMINIC ACID | IC50 | = | 68000.0 | nM | 4.17 |