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Assay Detail

CHEMBL5230040

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS stimulation and measured after 20 hrs by Griess reagent based assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Anti-inflammatory Quinoline Alkaloids from the Roots of <i>Waltheria indica</i>.
Liu F, O'Donnell TJ, Park EJ, Kovacs S, Nakamura K, Dave A, Luo Y, Sun R, Wall M, Wongwiwatthananukit S, Silva DK, Williams PG, Pezzuto JM, Chang LC.
J Nat Prod (2023) 86 : 276 -289
PubMed 36746775 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 4.62 4.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3342751 1 4.96
CHEMBL5291228 1 4.94
CHEMBL4743230 1 4.93
CHEMBL5288691 1 4.89
CHEMBL3342756 1 4.79
CHEMBL5267196 1 4.73
CHEMBL5281119 1 4.68
CHEMBL1744478 1 4.64
CHEMBL5281553 1 4.59
CHEMBL5283486 1 4.59
CHEMBL5284333 1 4.59
CHEMBL5272581 1 4.51
CHEMBL5286630 1 4.50
CHEMBL5285765 1 4.45
CHEMBL3105822 1 4.45
CHEMBL5274355 1 4.41
CHEMBL4785040 1 4.39
CHEMBL3342749 1 4.37
CHEMBL5289193 1 4.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3342751 IC50 = 11000.0 nM 4.96
CHEMBL5291228 IC50 = 11400.0 nM 4.94
CHEMBL4743230 IC50 = 11700.0 nM 4.93
CHEMBL5288691 IC50 = 12800.0 nM 4.89
CHEMBL3342756 IC50 = 16100.0 nM 4.79
CHEMBL5267196 IC50 = 18800.0 nM 4.73
CHEMBL5281119 IC50 = 20800.0 nM 4.68
CHEMBL1744478 IC50 = 22700.0 nM 4.64
CHEMBL5281553 IC50 = 25400.0 nM 4.59
CHEMBL5283486 IC50 = 25800.0 nM 4.59
CHEMBL5284333 IC50 = 25800.0 nM 4.59
CHEMBL5272581 IC50 = 31000.0 nM 4.51
CHEMBL5286630 IC50 = 32000.0 nM 4.50
CHEMBL5285765 IC50 = 35600.0 nM 4.45
CHEMBL3105822 IC50 = 35700.0 nM 4.45
CHEMBL5274355 IC50 = 38800.0 nM 4.41
CHEMBL4785040 IC50 = 40700.0 nM 4.39
CHEMBL3342749 IC50 = 42900.0 nM 4.37
CHEMBL5289193 IC50 = 43000.0 nM 4.37