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Assay Detail

CHEMBL5236743

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N terminal histidine tagged human recombinant ROCK1 (3 to 543 residues) in HASMC cells incubated for 2 hrs by ELISA method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HASMC
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Rho-associated protein kinase 1 (CHEMBL3231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Furazans in Medicinal Chemistry.
Mancini RS, Barden CJ, Weaver DF, Reed MA.
J Med Chem (2021) 64 : 1786 -1815
PubMed 33569941 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.19 5.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL220241 GSK-269962A 1 5.52
CHEMBL1956071 1 5.52
CHEMBL559147 Y-27632 1 4.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL220241 GSK-269962A IC50 = 3000.0 nM 5.52
CHEMBL1956071 IC50 = 3000.0 nM 5.52
CHEMBL559147 Y-27632 IC50 = 30000.0 nM 4.52