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Assay Detail

CHEMBL5239771

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FGFR4 (unknown origin) using TK as substrate incubated for 1 hr in presence of biotin/ATP by HTRF assay
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fibroblast growth factor receptor 4 (CHEMBL3973)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2-Amino-7-sulfonyl-7<i>H</i>-pyrrolo[2,3-<i>d</i>]pyrimidine Derivatives as Potent Reversible FGFR Inhibitors with Gatekeeper Mutation Tolerance: Design, Synthesis, and Biological Evaluation.
Xie W, Yang S, Liang L, Wang M, Zuo W, Lei Y, Zhang Y, Tang W, Lu T, Chen Y, Jiang Y.
J Med Chem (2022) 65 : 16570 -16588
PubMed 36480917 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 7.06 8.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4514636 FISOGATINIB 2.0 1 8.28
CHEMBL5290554 1 8.06
CHEMBL5281307 1 7.98
CHEMBL5283096 1 7.90
CHEMBL5268351 1 7.86
CHEMBL5280802 1 7.79
CHEMBL5272254 1 7.77
CHEMBL5278656 1 7.77
CHEMBL5268564 1 7.54
CHEMBL5275506 1 7.53
CHEMBL5280110 1 7.48
CHEMBL5267668 1 7.47
CHEMBL5279776 1 7.43
CHEMBL5283169 1 7.38
CHEMBL5289886 1 7.32
CHEMBL5290407 1 7.26
CHEMBL5267788 1 7.22
CHEMBL5276408 1 7.13
CHEMBL5283222 1 6.95
CHEMBL5265906 1 6.94
CHEMBL5274760 1 6.90
CHEMBL5288254 1 6.88
CHEMBL5273517 1 6.70
CHEMBL5290895 1 6.46
CHEMBL5286749 1 6.21
CHEMBL5274573 1 6.15
CHEMBL5273832 1 6.05
CHEMBL5291099 1 5.87
CHEMBL5289858 1 5.70
CHEMBL5269498 1 5.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4514636 FISOGATINIB IC50 = 5.3 nM 8.28
CHEMBL5290554 IC50 = 8.7 nM 8.06
CHEMBL5281307 IC50 = 10.4 nM 7.98
CHEMBL5283096 IC50 = 12.7 nM 7.90
CHEMBL5268351 IC50 = 13.9 nM 7.86
CHEMBL5280802 IC50 = 16.1 nM 7.79
CHEMBL5272254 IC50 = 17.1 nM 7.77
CHEMBL5278656 IC50 = 17.1 nM 7.77
CHEMBL5268564 IC50 = 29.0 nM 7.54
CHEMBL5275506 IC50 = 29.5 nM 7.53
CHEMBL5280110 IC50 = 33.0 nM 7.48
CHEMBL5267668 IC50 = 33.6 nM 7.47
CHEMBL5279776 IC50 = 36.8 nM 7.43
CHEMBL5283169 IC50 = 41.8 nM 7.38
CHEMBL5289886 IC50 = 47.5 nM 7.32
CHEMBL5290407 IC50 = 55.0 nM 7.26
CHEMBL5267788 IC50 = 60.5 nM 7.22
CHEMBL5276408 IC50 = 74.7 nM 7.13
CHEMBL5283222 IC50 = 111.7 nM 6.95
CHEMBL5265906 IC50 = 113.9 nM 6.94
CHEMBL5274760 IC50 = 127.0 nM 6.90
CHEMBL5288254 IC50 = 132.0 nM 6.88
CHEMBL5273517 IC50 = 197.9 nM 6.70
CHEMBL5290895 IC50 = 346.0 nM 6.46
CHEMBL5286749 IC50 = 614.5 nM 6.21
CHEMBL5274573 IC50 = 703.2 nM 6.15
CHEMBL5273832 IC50 = 892.7 nM 6.05
CHEMBL5291099 IC50 = 1343.0 nM 5.87
CHEMBL5289858 IC50 = 1975.0 nM 5.70
CHEMBL5269498 IC50 = 2195.0 nM 5.66
CHEMBL5269015 IC50 = 4770.0 nM 5.32
CHEMBL5288549 IC50 > 10000.0 nM -