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Assay Detail

CHEMBL5239853

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to saturated human muscarinic M1 expressed in CHO cells assessed as dissociation constant incubated for 2 hrs in presence of atropine by flow cytometry
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M1 (CHEMBL216)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, Characterization, and Application of Muscarinergic M<sub>3</sub> Receptor Ligands Linked to Fluorescent Dyes.
Köckenberger J, Fischer O, Konopa A, Bergwinkl S, Mühlich S, Gmeiner P, Kutta RJ, Hübner H, Keller M, Heinrich MR.
J Med Chem (2022) 65 : 16494 -16509
PubMed 36484801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 2 8.14 8.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5271406 1 8.19
CHEMBL5285607 1 8.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5271406 Kd = 6.5 nM 8.19
CHEMBL5285607 Kd = 8.3 nM 8.08