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Assay Detail

CHEMBL5260674

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human full length PI5P4Kalpha expressed in Escherichia coli BL21(DE3) pre-incubated for 15 mins followed by ATP addition measured after 1 hr by TR-FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Identification of ARUK2002821 as an isoform-selective PI5P4Kα inhibitor.
Willems HMG, Edwards S, Boffey HK, Chawner SJ, Green C, Romero T, Winpenny D, Skidmore J, Clarke JH, Andrews SP.
RSC Med Chem (2023) 14 : 934 -946
PubMed 37252102 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 4.75 4.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2104863 IMANIXIL 2.0 1 4.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2104863 IMANIXIL IC50 = 18000.0 nM 4.75