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Assay Detail

CHEMBL5320169

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human MC4R stably expressed in human Flp-In-293 cells assessed as cAMP accumulation incubated for 45 mins in presence of IBMX by Lance Ultra cAMP assay
16
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Flp-In-293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL259)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Targeting Melanocortin Receptors Using S<sub><i>N</i></sub>Ar-Type Macrocyclization: A Doubly Orthogonal Route to Cyclic Peptide Conjugates.
Yue WK, Zhang T, Shandre Mugan R, Barlow N, Chalmers DK, Pouton CW, Thompson PE.
J Med Chem (2023) 66 : 3273 -3283
PubMed 36808973 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 8.43 9.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5414451 1 9.87
CHEMBL430239 MELATONAN 1 9.70
CHEMBL5432868 1 9.56
CHEMBL5399539 1 9.35
CHEMBL5398659 1 9.30
CHEMBL5416095 2 9.01
CHEMBL5421885 1 9.00
CHEMBL5426684 1 8.80
CHEMBL5421108 1 8.30
CHEMBL5432664 1 8.20
CHEMBL5405038 1 7.70
CHEMBL5438253 1 7.20
CHEMBL5413092 1 7.05
CHEMBL5438629 1 6.50
CHEMBL504986 1 6.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5414451 EC50 = 0.1349 nM 9.87
CHEMBL430239 MELATONAN EC50 = 0.1995 nM 9.70
CHEMBL5432868 EC50 = 0.2754 nM 9.56
CHEMBL5399539 EC50 = 0.4467 nM 9.35
CHEMBL5398659 EC50 = 0.5012 nM 9.30
CHEMBL5416095 EC50 = 0.98 nM 9.01
CHEMBL5421885 EC50 = 1.0 nM 9.00
CHEMBL5416095 EC50 = 1.0 nM 9.00
CHEMBL5426684 EC50 = 1.585 nM 8.80
CHEMBL5421108 EC50 = 5.012 nM 8.30
CHEMBL5432664 EC50 = 6.31 nM 8.20
CHEMBL5405038 EC50 = 19.95 nM 7.70
CHEMBL5438253 EC50 = 63.1 nM 7.20
CHEMBL5413092 EC50 = 89.13 nM 7.05
CHEMBL5438629 EC50 = 316.23 nM 6.50
CHEMBL504986 EC50 = 501.19 nM 6.30