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Assay Detail

CHEMBL5323217

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length SARS-CoV-2 3CL protease expressed in Escherichia coli BL21 (DE3) using MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 as substrate preincubated for 10 mins followed by substrate addition and measured every 35 secs for 3.5 mins by FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome coronavirus 2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL4523582)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome coronavirus 2

Publication

Aurones: A Promising Scaffold to Inhibit SARS-CoV-2 Replication.
Caleffi GS, Rosa AS, de Souza LG, Avelar JLS, Nascimento SMR, de Almeida VM, Tucci AR, Ferreira VN, da Silva AJM, Santos-Filho OA, Miranda MD, Costa PRR.
J Nat Prod (2023) 86 : 1536 -1549
PubMed 37257024 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 6.05 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL8260 BAICALEIN 2.0 1 6.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL8260 BAICALEIN IC50 = 900.0 nM 6.05