Assay Detail
Binding
CHEMBL5325965
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human DNA polymerase eta (1 to 437 residues) using 5'-TTT TTT TTG C-TAMRA-3' reporter/5'-(FAM-TTT)-GGGGGAAGGATTC-3' primer/5'-TCACGGAATCCTTCCCCC-3' template as DNA substrate preincubated for 5 to 10 mins followed by DNA substrate addition measured immediately in presence of dTTP/MgCl2 by fluorescence based assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Computer-aided identification, synthesis, and biological evaluation of DNA polymerase η inhibitors for the treatment of cancer.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 5.10 | 5.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5433463 | — | — | 1 | 5.10 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5433463 | — | IC50 | = | 8000.0 | nM | 5.10 |