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Assay Detail

CHEMBL5330540

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]pyrilamine from Sprague-Dawley rat H1 receptor assessed as inhibition constant measured after 30 mins by competitive binding assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL4701)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

New aryl-/heteroarylpiperazine derivatives of 1,7-dimethyl-8,9-diphenyl-4-azatricyclo[5.2.1.0<sup>2,6</sup>]dec-8-ene-3,5,10-trione: Synthesis and preliminary studies of biological activities.
Napiórkowska M, Kurpios-Piec D, Kiernozek-Kalińska E, Leśniak A, Klawikowska M, Bujalska-Zadrożny M.
Bioorg Med Chem (2023) 96 : 117518 -117518
PubMed 37951135 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 7.53 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL643 PROMETHAZINE 4.0 1 9.00
CHEMBL5403228 1 8.22
CHEMBL5395179 1 8.15
CHEMBL5435378 1 7.52
CHEMBL5409943 1 7.10
CHEMBL5422403 1 6.52
CHEMBL5437394 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL643 PROMETHAZINE Ki = 1.0 nM 9.00
CHEMBL5403228 Ki = 6.0 nM 8.22
CHEMBL5395179 Ki = 7.0 nM 8.15
CHEMBL5435378 Ki = 30.0 nM 7.52
CHEMBL5409943 Ki = 80.0 nM 7.10
CHEMBL5422403 Ki = 300.0 nM 6.52
CHEMBL5437394 Ki = 600.0 nM 6.22