Assay Detail
Binding
CHEMBL5336348
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal His6-tagged recombinant full length SARS-CoV-2 3CLpro expressed in Escherichia coli BL21(DE3) using (KTSAVLQSGFRKME) as fluorogenic substrate preincubated with compound for 10 mins followed by substrate addition by FRET assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1ab (CHEMBL4523582) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome coronavirus 2 |
Publication
The research progress of SARS-CoV-2 main protease inhibitors from 2020 to 2022.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 5.38 | 6.58 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1096283 | — | — | 1 | 6.58 |
| CHEMBL413376 | SURAMIN HEXASODIUM | 3.0 | 1 | 5.19 |
| CHEMBL5402071 | — | — | 1 | 5.17 |
| CHEMBL2380760 | — | — | 1 | 5.01 |
| CHEMBL5271003 | — | — | 1 | 4.94 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1096283 | — | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL413376 | SURAMIN HEXASODIUM | IC50 | = | 6500.0 | nM | 5.19 |
| CHEMBL5402071 | — | IC50 | = | 6810.0 | nM | 5.17 |
| CHEMBL2380760 | — | IC50 | = | 9840.0 | nM | 5.01 |
| CHEMBL5271003 | — | IC50 | = | 11390.0 | nM | 4.94 |