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Assay Detail

CHEMBL5336802

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mouse GAT2 transfected in HEK293 cells assessed as reduction in [3H]-GABA uptake preincubated for 25 mins followed by [3H]-GABA addition and measured after 10 mins by liquid scintillation counting analysis
30
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

GABA transporter 2 (CHEMBL4228)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of novel multifunctional ligands targeting GABA transporters, butyrylcholinesterase, β-secretase, and amyloid β aggregation as potential treatment of Alzheimer's disease.
Zaręba P, Łątka K, Mazur G, Gryzło B, Pasieka A, Godyń J, Panek D, Skrzypczak-Wiercioch A, Höfner GC, Latacz G, Maj M, Espargaró A, Sabaté R, Jóźwiak K, Wanner KT, Sałat K, Malawska B, Kulig K, Bajda M.
Eur J Med Chem (2023) 261 : 115832 -115832
PubMed 37837674 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 4.89 5.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5411369 2 5.52
CHEMBL5423337 1 5.18
CHEMBL5409309 1 5.11
CHEMBL5416293 1 5.08
CHEMBL5409538 1 5.02
CHEMBL5403788 2 5.01
CHEMBL5411905 1 4.99
CHEMBL5423441 1 4.96
CHEMBL5407267 1 4.94
CHEMBL5423316 1 4.91
CHEMBL5419107 1 4.89
CHEMBL5412199 1 4.89
CHEMBL5394533 2 4.87
CHEMBL5423098 1 4.86
CHEMBL5405236 1 4.85
CHEMBL5424877 2 4.82
CHEMBL5440565 1 4.80
CHEMBL5435638 1 4.79
CHEMBL5398245 1 4.72
CHEMBL5438498 1 4.70
CHEMBL5425025 1 4.70
CHEMBL5414446 1 4.69
CHEMBL5397274 1 4.65
CHEMBL5413754 1 4.61
CHEMBL5416743 1 4.57
CHEMBL5397616 1 4.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5411369 IC50 = 3000.0 nM 5.52
CHEMBL5411369 IC50 = 3090.3 nM 5.51
CHEMBL5423337 IC50 = 6606.93 nM 5.18
CHEMBL5409309 IC50 = 7762.47 nM 5.11
CHEMBL5416293 IC50 = 8317.64 nM 5.08
CHEMBL5409538 IC50 = 9549.93 nM 5.02
CHEMBL5403788 IC50 = 9772.37 nM 5.01
CHEMBL5411905 IC50 = 10232.93 nM 4.99
CHEMBL5423441 IC50 = 10964.78 nM 4.96
CHEMBL5407267 IC50 = 11481.54 nM 4.94
CHEMBL5403788 IC50 = 11748.98 nM 4.93
CHEMBL5423316 IC50 = 12302.69 nM 4.91
CHEMBL5412199 IC50 = 12882.5 nM 4.89
CHEMBL5419107 IC50 = 12882.5 nM 4.89
CHEMBL5394533 IC50 = 13489.63 nM 4.87
CHEMBL5423098 IC50 = 13803.84 nM 4.86
CHEMBL5405236 IC50 = 14125.38 nM 4.85
CHEMBL5424877 IC50 = 15135.61 nM 4.82
CHEMBL5394533 IC50 = 15488.17 nM 4.81
CHEMBL5440565 IC50 = 15848.93 nM 4.80
CHEMBL5435638 IC50 = 16218.1 nM 4.79
CHEMBL5424877 IC50 = 18197.01 nM 4.74
CHEMBL5398245 IC50 = 19054.61 nM 4.72
CHEMBL5438498 IC50 = 19952.62 nM 4.70
CHEMBL5425025 IC50 = 19952.62 nM 4.70
CHEMBL5414446 IC50 = 20417.38 nM 4.69
CHEMBL5397274 IC50 = 22387.21 nM 4.65
CHEMBL5413754 IC50 = 24547.09 nM 4.61
CHEMBL5416743 IC50 = 26915.35 nM 4.57
CHEMBL5397616 IC50 = 31622.78 nM 4.50