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Assay Detail

CHEMBL5338147

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition and measured every min for 10 mins by Ellman's method
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Equus caballus
Tissue Serum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL5763)
Type SINGLE PROTEIN
Organism Equus caballus

Publication

Design, synthesis and preliminary biological evaluation of rivastigmine-INDY hybrids as multitarget ligands against Alzheimer's disease by targeting butyrylcholinesterase and DYRK1A/CLK1 kinases.
Ţînţaş ML, Peauger L, Barré A, Papamicaël C, Besson T, Sopkovà-de Oliveira Santos J, Gembus V, Levacher V.
RSC Med Chem (2024) 15 : 963 -980
PubMed 38516603 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.23 8.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL95 TACRINE 4.0 1 8.72
CHEMBL5402840 1 7.57
CHEMBL5396284 1 7.17
CHEMBL5417812 1 6.81
CHEMBL5408824 1 6.36
CHEMBL5402721 1 6.33
CHEMBL5408141 1 6.15
CHEMBL5397089 1 6.12
CHEMBL5398338 1 6.09
CHEMBL5398767 1 5.79
CHEMBL5407180 1 5.70
CHEMBL5428725 1 5.66
CHEMBL5407164 1 5.22
CHEMBL5434888 1 4.92
CHEMBL5414144 1 4.89
CHEMBL5440931 1 -
CHEMBL5412852 1 -
CHEMBL5400482 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL95 TACRINE IC50 = 1.9 nM 8.72
CHEMBL5402840 IC50 = 26.7 nM 7.57
CHEMBL5396284 IC50 = 68.0 nM 7.17
CHEMBL5417812 IC50 = 154.0 nM 6.81
CHEMBL5408824 IC50 = 439.0 nM 6.36
CHEMBL5402721 IC50 = 470.0 nM 6.33
CHEMBL5408141 IC50 = 703.0 nM 6.15
CHEMBL5397089 IC50 = 761.0 nM 6.12
CHEMBL5398338 IC50 = 821.0 nM 6.09
CHEMBL5398767 IC50 = 1640.0 nM 5.79
CHEMBL5407180 IC50 = 2000.0 nM 5.70
CHEMBL5428725 IC50 = 2195.0 nM 5.66
CHEMBL5407164 IC50 = 6000.0 nM 5.22
CHEMBL5434888 IC50 = 12000.0 nM 4.92
CHEMBL5414144 IC50 = 13000.0 nM 4.89
CHEMBL5440931 IC50 > 10000.0 nM -
CHEMBL5412852 IC50 > 10000.0 nM -
CHEMBL5400482 IC50 > 10000.0 nM -