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Assay Detail

CHEMBL5345753

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SIK3 isoform 1 (59 to 1321 residues) expressed in Sf9 insect cells using AMARA peptide as substrate incubated for 120 mins in presence of ATP by ADP-Glo assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase SIK3 (CHEMBL6149)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of Selectivity and Pharmacokinetic Properties of Salt-Inducible Kinase Inhibitors that Led to the Discovery of Pan-SIK Inhibitor GLPG3312.
Temal-Laib T, Peixoto C, Desroy N, De Lemos E, Bonnaterre F, Bienvenu N, Picolet O, Sartori E, Bucher D, López-Ramos M, Roca Magadán C, Laenen W, Flower T, Mollat P, Bugaud O, Touitou R, Pereira Fernandes A, Lavazais S, Monjardet A, Borgonovi M, Gosmini R, Brys R, Amantini D, De Vos S, Andrews M.
J Med Chem (2024) 67 : 380 -401
PubMed 38147525 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 8.30 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5417444 1 9.22
CHEMBL5438171 1 9.00
CHEMBL5431938 1 9.00
CHEMBL5415380 1 8.96
CHEMBL5412961 1 8.89
CHEMBL5404502 1 8.85
CHEMBL5397288 1 8.66
CHEMBL5401167 1 8.54
CHEMBL5427025 1 8.43
CHEMBL5420455 1 8.38
CHEMBL5396179 1 8.38
CHEMBL5403558 1 8.36
CHEMBL5415699 1 8.30
CHEMBL5419476 1 8.18
CHEMBL5395705 1 8.17
CHEMBL5430774 1 7.99
CHEMBL5428147 1 7.91
CHEMBL5428443 1 7.28
CHEMBL5410107 1 6.79
CHEMBL5417490 1 6.73
CHEMBL5400669 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5417444 IC50 = 0.6 nM 9.22
CHEMBL5438171 IC50 = 1.0 nM 9.00
CHEMBL5431938 IC50 = 1.0 nM 9.00
CHEMBL5415380 IC50 = 1.1 nM 8.96
CHEMBL5412961 IC50 = 1.3 nM 8.89
CHEMBL5404502 IC50 = 1.4 nM 8.85
CHEMBL5397288 IC50 = 2.2 nM 8.66
CHEMBL5401167 IC50 = 2.9 nM 8.54
CHEMBL5427025 IC50 = 3.7 nM 8.43
CHEMBL5420455 IC50 = 4.2 nM 8.38
CHEMBL5396179 IC50 = 4.2 nM 8.38
CHEMBL5403558 IC50 = 4.4 nM 8.36
CHEMBL5415699 IC50 = 5.0 nM 8.30
CHEMBL5419476 IC50 = 6.6 nM 8.18
CHEMBL5395705 IC50 = 6.8 nM 8.17
CHEMBL5430774 IC50 = 10.2 nM 7.99
CHEMBL5428147 IC50 = 12.3 nM 7.91
CHEMBL5428443 IC50 = 53.1 nM 7.28
CHEMBL5410107 IC50 = 163.7 nM 6.79
CHEMBL5417490 IC50 = 188.0 nM 6.73
CHEMBL5400669 IC50 > 4000.0 nM -