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Assay Detail

CHEMBL5353064

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human URAT1 stably overexpressing in human HEK293 cells assessed as inhibition of 14C-uric acid uptake preincubated for 30 mins followed by substrate addition and measured after 15 mins using 14C-uric acid as substrate by liquid scintillation counting analysis
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Solute carrier family 22 member 12 (CHEMBL6120)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel benzbromarone analogs with improved pharmacokinetics and benign toxicity profiles as antihyperuricemic agents.
Zhao Z, Liu J, Yuan L, Yang Z, Kuang P, Liao H, Luo J, Feng H, Zheng F, Chen Y, Wu T, Guo J, Cao Y, Yang Y, Lin C, Zhang Q, Chen J, Pang J.
Eur J Med Chem (2022) 242 : 114682 -114682
PubMed 36001935 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 5.70 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3707347 VERINURAD 2.0 1 6.89
CHEMBL388590 BENZBROMARONE 4.0 1 6.28
CHEMBL5439993 1 6.10
CHEMBL5405072 1 5.81
CHEMBL5400167 1 5.81
CHEMBL5429502 1 5.80
CHEMBL5396194 1 5.80
CHEMBL5395589 1 5.76
CHEMBL5417202 1 5.74
CHEMBL5437905 1 5.70
CHEMBL5412709 1 5.68
CHEMBL5397658 1 5.65
CHEMBL5405794 1 5.64
CHEMBL5438015 1 5.63
CHEMBL5436610 1 5.57
CHEMBL5417306 1 5.57
CHEMBL5435294 1 5.55
CHEMBL5421730 1 5.53
CHEMBL5437838 1 5.49
CHEMBL5396907 1 5.42
CHEMBL5394644 1 5.31
CHEMBL5426916 1 5.23
CHEMBL2105720 LESINURAD 4.0 1 5.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3707347 VERINURAD IC50 = 130.0 nM 6.89
CHEMBL388590 BENZBROMARONE IC50 = 530.0 nM 6.28
CHEMBL5439993 IC50 = 800.0 nM 6.10
CHEMBL5405072 IC50 = 1560.0 nM 5.81
CHEMBL5400167 IC50 = 1550.0 nM 5.81
CHEMBL5429502 IC50 = 1600.0 nM 5.80
CHEMBL5396194 IC50 = 1580.0 nM 5.80
CHEMBL5395589 IC50 = 1720.0 nM 5.76
CHEMBL5417202 IC50 = 1810.0 nM 5.74
CHEMBL5437905 IC50 = 1980.0 nM 5.70
CHEMBL5412709 IC50 = 2080.0 nM 5.68
CHEMBL5397658 IC50 = 2260.0 nM 5.65
CHEMBL5405794 IC50 = 2300.0 nM 5.64
CHEMBL5438015 IC50 = 2360.0 nM 5.63
CHEMBL5436610 IC50 = 2700.0 nM 5.57
CHEMBL5417306 IC50 = 2690.0 nM 5.57
CHEMBL5435294 IC50 = 2810.0 nM 5.55
CHEMBL5421730 IC50 = 2950.0 nM 5.53
CHEMBL5437838 IC50 = 3220.0 nM 5.49
CHEMBL5396907 IC50 = 3800.0 nM 5.42
CHEMBL5394644 IC50 = 4850.0 nM 5.31
CHEMBL5426916 IC50 = 5830.0 nM 5.23
CHEMBL2105720 LESINURAD IC50 = 7560.0 nM 5.12