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Assay Detail

CHEMBL5356226

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kalpha (unknown origin) using PIP2 as substrate incubated for 1 hr in presence of ATP by Kinase-Glo plus luminescent assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Function-oriented synthesis of Imidazo[1,2-a]pyrazine and Imidazo[1,2-b]pyridazine derivatives as potent PI3K/mTOR dual inhibitors.
Li C, Han Y, Wang Z, Yu Y, Wang C, Ren Z, Guo Y, Zhu T, Li X, Dong S, Ma M.
Eur J Med Chem (2023) 247 : 115030 -115030
PubMed 36586298 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 8.97 10.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5395998 1 10.22
CHEMBL5422635 1 10.10
CHEMBL3218576 COPANLISIB 4.0 1 9.96
CHEMBL5400439 1 9.96
CHEMBL5401298 1 9.80
CHEMBL5413309 1 9.80
CHEMBL5415726 1 9.77
CHEMBL4440406 1 9.70
CHEMBL5431325 1 9.62
CHEMBL5409142 1 9.62
CHEMBL4213353 1 9.51
CHEMBL5428160 1 9.34
CHEMBL5440536 1 9.19
CHEMBL5415865 1 9.00
CHEMBL5432324 1 8.96
CHEMBL5428411 1 8.94
CHEMBL5417402 1 8.88
CHEMBL5431132 1 8.44
CHEMBL5398514 1 8.28
CHEMBL5402186 1 8.18
CHEMBL5403717 1 8.14
CHEMBL5407573 1 8.13
CHEMBL5429174 1 8.12
CHEMBL5401801 1 8.04
CHEMBL5421477 1 7.98
CHEMBL5394535 1 7.95
CHEMBL5405706 1 7.95
CHEMBL5405623 1 7.44
CHEMBL5418523 1 -
CHEMBL1765463 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5395998 IC50 = 0.06 nM 10.22
CHEMBL5422635 IC50 = 0.08 nM 10.10
CHEMBL3218576 COPANLISIB IC50 = 0.11 nM 9.96
CHEMBL5400439 IC50 = 0.11 nM 9.96
CHEMBL5401298 IC50 = 0.16 nM 9.80
CHEMBL5413309 IC50 = 0.16 nM 9.80
CHEMBL5415726 IC50 = 0.17 nM 9.77
CHEMBL4440406 IC50 = 0.2 nM 9.70
CHEMBL5431325 IC50 = 0.24 nM 9.62
CHEMBL5409142 IC50 = 0.24 nM 9.62
CHEMBL4213353 IC50 = 0.31 nM 9.51
CHEMBL5428160 IC50 = 0.46 nM 9.34
CHEMBL5440536 IC50 = 0.65 nM 9.19
CHEMBL5415865 IC50 = 0.99 nM 9.00
CHEMBL5432324 IC50 = 1.09 nM 8.96
CHEMBL5428411 IC50 = 1.16 nM 8.94
CHEMBL5417402 IC50 = 1.33 nM 8.88
CHEMBL5431132 IC50 = 3.62 nM 8.44
CHEMBL5398514 IC50 = 5.21 nM 8.28
CHEMBL5402186 IC50 = 6.63 nM 8.18
CHEMBL5403717 IC50 = 7.2 nM 8.14
CHEMBL5407573 IC50 = 7.46 nM 8.13
CHEMBL5429174 IC50 = 7.53 nM 8.12
CHEMBL5401801 IC50 = 9.01 nM 8.04
CHEMBL5421477 IC50 = 10.4 nM 7.98
CHEMBL5394535 IC50 = 11.2 nM 7.95
CHEMBL5405706 IC50 = 11.3 nM 7.95
CHEMBL5405623 IC50 = 36.5 nM 7.44
CHEMBL5418523 IC50 > 5.0 nM -
CHEMBL1765463 IC50 > 5.0 nM -