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Assay Detail

CHEMBL5362071

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse LPS1/GPR34 transfected in HEK293-A cells co-transfected with AP-TGFalpha assessed as AP-TGFalpha release by TGFalpha shedding assay
22
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293-A
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Probable G-protein coupled receptor 34 (CHEMBL1075291)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Exploration of LPS<sub>2</sub> agonist binding modes using the combination of a new hydrophobic scaffold and homology modeling.
Chen L, Uwamizu A, Sayama M, Kano K, Otani Y, Kondo S, Inoue A, Aoki J, Ohwada T.
Eur J Med Chem (2023) 252 : 115271 -115271
PubMed 36965226 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 21 6.35 6.75
pEC50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5410755 2 6.75
CHEMBL4072692 2 6.66
CHEMBL1742484 2 6.54
CHEMBL5398282 2 6.02
CHEMBL5393782 2 5.80
CHEMBL5404040 2 -
CHEMBL5394475 2 -
CHEMBL4099594 2 -
CHEMBL5432264 2 -
CHEMBL5398524 2 -
CHEMBL5428143 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5410755 EC50 = 180.0 nM 6.75
CHEMBL5410755 EC50 = 177.83 nM 6.75
CHEMBL4072692 EC50 = 218.78 nM 6.66
CHEMBL4072692 EC50 = 220.0 nM 6.66
CHEMBL1742484 EC50 = 290.0 nM 6.54
CHEMBL1742484 EC50 = 295.12 nM 6.53
CHEMBL5398282 EC50 = 950.0 nM 6.02
CHEMBL5398282 EC50 = 954.99 nM 6.02
CHEMBL5393782 EC50 = 1600.0 nM 5.80
CHEMBL5393782 EC50 = 1621.81 nM 5.79
CHEMBL5404040 EC50 > 3000.0 nM -
CHEMBL5428143 pEC50 - -
CHEMBL5398524 EC50 < 3162.28 nM -
CHEMBL5432264 EC50 < 3162.28 nM -
CHEMBL4099594 EC50 < 3162.28 nM -
CHEMBL5394475 EC50 < 3162.28 nM -
CHEMBL5404040 EC50 < 3162.28 nM -
CHEMBL5394475 EC50 > 3000.0 nM -
CHEMBL4099594 EC50 > 3000.0 nM -
CHEMBL5428143 EC50 - -
CHEMBL5398524 EC50 > 3000.0 nM -
CHEMBL5432264 EC50 > 3000.0 nM -