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Assay Detail

CHEMBL5362824

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST-tagged human recombinant TRKA G595R mutant (440 to end residues) expressed in baculovirus infected Sf9 insect cells incubated for 30 mins in presence of ATP by HTRF assay
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

High affinity nerve growth factor receptor (CHEMBL2815)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of novel indolin-2-one derivatives as potent second-generation TRKs inhibitors.
Qin Q, Fu Q, Wang X, Lv R, Lu S, Guo Z, Wu T, Sun Y, Sun Y, Liu N, Zhao D, Cheng M.
Eur J Med Chem (2023) 253 : 115291 -115291
PubMed 37030091 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 6.13 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4297627 SELITRECTINIB 2.0 1 8.10
CHEMBL5431992 1 8.00
CHEMBL5402022 1 7.85
CHEMBL5412694 1 7.44
CHEMBL5411653 1 6.80
CHEMBL5405520 1 6.52
CHEMBL5403006 1 6.51
CHEMBL2037226 1 6.50
CHEMBL5412950 1 6.47
CHEMBL5429071 1 6.44
CHEMBL1983268 ENTRECTINIB 4.0 1 6.40
CHEMBL5426432 1 6.27
CHEMBL5435508 1 6.27
CHEMBL5421895 1 6.22
CHEMBL5416004 1 6.20
CHEMBL5436311 1 6.19
CHEMBL5424554 1 6.11
CHEMBL5412491 1 6.09
CHEMBL5402630 1 6.09
CHEMBL5393854 1 6.07
CHEMBL5409228 1 5.97
CHEMBL5393979 1 5.87
CHEMBL5434909 1 5.79
CHEMBL5422996 1 5.73
CHEMBL5418831 1 5.72
CHEMBL5415584 1 5.69
CHEMBL5429205 1 5.68
CHEMBL5430182 1 5.64
CHEMBL5406744 1 5.61
CHEMBL5406403 1 5.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4297627 SELITRECTINIB IC50 = 8.0 nM 8.10
CHEMBL5431992 IC50 = 10.0 nM 8.00
CHEMBL5402022 IC50 = 14.0 nM 7.85
CHEMBL5412694 IC50 = 36.0 nM 7.44
CHEMBL5411653 IC50 = 160.0 nM 6.80
CHEMBL5405520 IC50 = 300.0 nM 6.52
CHEMBL5403006 IC50 = 310.0 nM 6.51
CHEMBL2037226 IC50 = 320.0 nM 6.50
CHEMBL5412950 IC50 = 340.0 nM 6.47
CHEMBL5429071 IC50 = 360.0 nM 6.44
CHEMBL1983268 ENTRECTINIB IC50 = 400.0 nM 6.40
CHEMBL5426432 IC50 = 540.0 nM 6.27
CHEMBL5435508 IC50 = 540.0 nM 6.27
CHEMBL5421895 IC50 = 600.0 nM 6.22
CHEMBL5416004 IC50 = 630.0 nM 6.20
CHEMBL5436311 IC50 = 650.0 nM 6.19
CHEMBL5424554 IC50 = 780.0 nM 6.11
CHEMBL5412491 IC50 = 820.0 nM 6.09
CHEMBL5402630 IC50 = 820.0 nM 6.09
CHEMBL5393854 IC50 = 850.0 nM 6.07
CHEMBL5409228 IC50 = 1070.0 nM 5.97
CHEMBL5393979 IC50 = 1360.0 nM 5.87
CHEMBL5434909 IC50 = 1610.0 nM 5.79
CHEMBL5422996 IC50 = 1880.0 nM 5.73
CHEMBL5418831 IC50 = 1920.0 nM 5.72
CHEMBL5415584 IC50 = 2060.0 nM 5.69
CHEMBL5429205 IC50 = 2100.0 nM 5.68
CHEMBL5430182 IC50 = 2270.0 nM 5.64
CHEMBL5406744 IC50 = 2440.0 nM 5.61
CHEMBL5406403 IC50 = 2590.0 nM 5.59
CHEMBL5406562 IC50 = 3160.0 nM 5.50
CHEMBL5399429 IC50 = 3490.0 nM 5.46
CHEMBL5412443 IC50 = 3850.0 nM 5.42
CHEMBL5423414 IC50 = 4100.0 nM 5.39
CHEMBL5426605 IC50 = 4620.0 nM 5.33
CHEMBL5439400 IC50 = 9300.0 nM 5.03
CHEMBL5432702 IC50 = 13320.0 nM 4.88
CHEMBL5421856 IC50 - -
CHEMBL5404415 IC50 - -
CHEMBL5401510 IC50 - -