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Assay Detail

CHEMBL5368952

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP3A4 in human liver microsomes using midazolam as substrate preincubated for 5 mins followed by NADPH addition by LC-MS/MS analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Developing Novel Coumarin-Containing Azoles Antifungal Agents by the Scaffold Merging Strategy for Treating Azole-Resistant Candidiasis.
Yan Z, Huang Y, Zhao D, Li Z, Wang X, Guo M, Wei Y, Wang Y, Mou Y, Hou Z, Guo C.
J Med Chem (2023) 66 : 13247 -13265
PubMed 37725043 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.36 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL91 MICONAZOLE 4.0 1 7.52
CHEMBL5423031 1 7.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL91 MICONAZOLE IC50 = 30.0 nM 7.52
CHEMBL5423031 IC50 = 64.0 nM 7.19