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Assay Detail

CHEMBL5372118

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Positive allosteric modulator activity at human TGR5 expressed in HEK293T cells assessed as increase in cAMP accumulation incubated for 1 hr at 37 degC and further incubated for 1 hr at room temperature by glosensor cAMP accumulation assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled bile acid receptor 1 (CHEMBL5409)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and biological evaluation of cholic acid derivatives as potent TGR5 positive allosteric modulators.
Li Y, Sun J, Wang X, Luo Z, Shao X, Li Y, Cao Q, Zhao S, Qian M, Chen X.
Bioorg Med Chem (2023) 92 : 117418 -117418
PubMed 37536263 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 23 4.71 5.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5439396 1 5.28
CHEMBL5416364 1 5.26
CHEMBL5440059 1 5.20
CHEMBL5406515 1 5.10
CHEMBL5422935 1 5.05
CHEMBL5415850 1 5.02
CHEMBL5435987 1 5.01
CHEMBL5429083 1 4.92
CHEMBL5430487 1 4.85
CHEMBL5419988 1 4.73
CHEMBL5423954 1 4.70
CHEMBL5440151 1 4.66
CHEMBL5414606 1 4.64
CHEMBL5435030 1 4.59
CHEMBL5427709 1 4.50
CHEMBL5431401 1 4.42
CHEMBL205596 CHOLIC ACID 4.0 1 4.41
CHEMBL5395512 1 4.31
CHEMBL5422399 1 4.21
CHEMBL5401550 1 4.12
CHEMBL5440153 1 4.03
CHEMBL5395727 1 -
CHEMBL5398083 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5439396 EC50 = 5300.0 nM 5.28
CHEMBL5416364 EC50 = 5500.0 nM 5.26
CHEMBL5440059 EC50 = 6300.0 nM 5.20
CHEMBL5406515 EC50 = 8000.0 nM 5.10
CHEMBL5422935 EC50 = 8900.0 nM 5.05
CHEMBL5415850 EC50 = 9500.0 nM 5.02
CHEMBL5435987 EC50 = 9800.0 nM 5.01
CHEMBL5429083 EC50 = 12000.0 nM 4.92
CHEMBL5430487 EC50 = 14100.0 nM 4.85
CHEMBL5419988 EC50 = 18500.0 nM 4.73
CHEMBL5423954 EC50 = 19800.0 nM 4.70
CHEMBL5440151 EC50 = 21800.0 nM 4.66
CHEMBL5414606 EC50 = 23000.0 nM 4.64
CHEMBL5435030 EC50 = 25800.0 nM 4.59
CHEMBL5427709 EC50 = 31600.0 nM 4.50
CHEMBL5431401 EC50 = 38000.0 nM 4.42
CHEMBL205596 CHOLIC ACID EC50 = 38700.0 nM 4.41
CHEMBL5395512 EC50 = 48500.0 nM 4.31
CHEMBL5422399 EC50 = 62000.0 nM 4.21
CHEMBL5401550 EC50 = 76500.0 nM 4.12
CHEMBL5440153 EC50 = 92500.0 nM 4.03
CHEMBL5395727 EC50 > 100000.0 nM -
CHEMBL5398083 EC50 > 100000.0 nM -