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Assay Detail

CHEMBL5389546

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of AKT phosphorylation in human SK-BR-3 cells harboring wildtype PI3Kalpha incubated for 24 hrs by AlphaLISA assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-BR-3
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Serine/threonine-protein kinase AKT (CHEMBL2111353)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Discovery of Pyridopyrimidinones that Selectively Inhibit the H1047R PI3Kα Mutant Protein.
Ketcham JM, Harwood SJ, Aranda R, Aloiau AN, Bobek BM, Briere DM, Burns AC, Caddell Haatveit K, Calinisan A, Clarine J, Elliott A, Engstrom LD, Gunn RJ, Ivetac A, Jones B, Kuehler J, Lawson JD, Nguyen N, Parker C, Pearson KE, Rahbaek L, Saechao B, Wang X, Waters A, Waters L, Watkins AH, Olson P, Smith CR, Christensen JG, Marx MA.
J Med Chem (2024) 67 : 4936 -4949
PubMed 38477582 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.90 6.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5404456 1 6.94
CHEMBL5406950 1 6.25
CHEMBL5408522 1 6.17
CHEMBL5437679 1 6.11
CHEMBL5423227 1 5.83
CHEMBL5424195 1 5.74
CHEMBL5419204 1 5.50
CHEMBL5414256 1 5.32
CHEMBL5436038 1 5.23
CHEMBL5440814 1 -
CHEMBL5401780 1 -
CHEMBL5426352 1 -
CHEMBL5424735 1 -
CHEMBL5394975 1 -
CHEMBL5411976 1 -
CHEMBL5402470 1 -
CHEMBL5413908 1 -
CHEMBL5431134 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5404456 IC50 = 115.0 nM 6.94
CHEMBL5406950 IC50 = 567.0 nM 6.25
CHEMBL5408522 IC50 = 681.0 nM 6.17
CHEMBL5437679 IC50 = 779.0 nM 6.11
CHEMBL5423227 IC50 = 1465.0 nM 5.83
CHEMBL5424195 IC50 = 1803.0 nM 5.74
CHEMBL5419204 IC50 = 3185.0 nM 5.50
CHEMBL5414256 IC50 = 4739.0 nM 5.32
CHEMBL5436038 IC50 = 5894.0 nM 5.23
CHEMBL5440814 IC50 > 10000.0 nM -
CHEMBL5401780 IC50 > 10000.0 nM -
CHEMBL5426352 IC50 > 10000.0 nM -
CHEMBL5424735 IC50 > 10000.0 nM -
CHEMBL5394975 IC50 > 10000.0 nM -
CHEMBL5411976 IC50 > 10000.0 nM -
CHEMBL5402470 IC50 > 10000.0 nM -
CHEMBL5413908 IC50 > 10000.0 nM -
CHEMBL5431134 IC50 > 10000.0 nM -