Assay Detail
Binding
CHEMBL5442179
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: HTR1B - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 27 | 6.51 | 8.93 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5089005 | — | — | 1 | 8.93 |
| CHEMBL281350 | SB-224289 | — | 1 | 8.56 |
| CHEMBL2164327 | — | — | 1 | 7.99 |
| CHEMBL1256863 | — | — | 1 | 7.95 |
| CHEMBL2138684 | FROVATRIPTAN SUCCINATE | 4.0 | 1 | 7.64 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 7.37 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | 4.0 | 1 | 6.97 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | 4.0 | 1 | 6.89 |
| CHEMBL540612 | — | — | 1 | 6.78 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | — | 1 | 6.63 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | 4.0 | 1 | 6.58 |
| CHEMBL267777 | RITANSERIN | 2.0 | 1 | 6.55 |
| CHEMBL405355 | NIGULDIPINE | 2.0 | 1 | 6.44 |
| CHEMBL24778 | SILODOSIN | 4.0 | 1 | 6.43 |
| CHEMBL5075931 | — | — | 1 | 6.25 |
| CHEMBL103769 | — | — | 1 | 5.98 |
| CHEMBL4743495 | — | — | 1 | 5.94 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | 4.0 | 1 | 5.91 |
| CHEMBL4743049 | — | — | 1 | 5.78 |
| CHEMBL1256844 | — | — | 1 | 5.77 |
| CHEMBL1633 | KETOTIFEN FUMARATE | 4.0 | 1 | 5.76 |
| CHEMBL1263 | SALMETEROL | 4.0 | 1 | 5.70 |
| CHEMBL59898 | — | — | 1 | 5.67 |
| CHEMBL1489369 | — | — | 1 | 5.55 |
| CHEMBL1200809 | AZELASTINE HYDROCHLORIDE | 4.0 | 1 | 5.37 |
| CHEMBL4202825 | — | — | 1 | 5.18 |
| CHEMBL403225 | — | — | 1 | 5.08 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5089005 | — | Ki | = | 1.183 | nM | 8.93 |
| CHEMBL281350 | SB-224289 | Ki | = | 2.777 | nM | 8.56 |
| CHEMBL2164327 | — | Ki | = | 10.2 | nM | 7.99 |
| CHEMBL1256863 | — | Ki | = | 11.16 | nM | 7.95 |
| CHEMBL2138684 | FROVATRIPTAN SUCCINATE | Ki | = | 22.87 | nM | 7.64 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 42.64 | nM | 7.37 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | Ki | = | 107.89 | nM | 6.97 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | Ki | = | 128.52 | nM | 6.89 |
| CHEMBL540612 | — | Ki | = | 166.0 | nM | 6.78 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | Ki | = | 232.27 | nM | 6.63 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | Ki | = | 262.12 | nM | 6.58 |
| CHEMBL267777 | RITANSERIN | Ki | = | 280.67 | nM | 6.55 |
| CHEMBL405355 | NIGULDIPINE | Ki | = | 365.64 | nM | 6.44 |
| CHEMBL24778 | SILODOSIN | Ki | = | 372.99 | nM | 6.43 |
| CHEMBL5075931 | — | Ki | = | 562.21 | nM | 6.25 |
| CHEMBL103769 | — | Ki | = | 1038.25 | nM | 5.98 |
| CHEMBL4743495 | — | Ki | = | 1156.91 | nM | 5.94 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | Ki | = | 1244.8 | nM | 5.91 |
| CHEMBL4743049 | — | Ki | = | 1662.65 | nM | 5.78 |
| CHEMBL1256844 | — | Ki | = | 1691.61 | nM | 5.77 |
| CHEMBL1633 | KETOTIFEN FUMARATE | Ki | = | 1753.07 | nM | 5.76 |
| CHEMBL1263 | SALMETEROL | Ki | = | 1985.41 | nM | 5.70 |
| CHEMBL59898 | — | Ki | = | 2157.0 | nM | 5.67 |
| CHEMBL1489369 | — | Ki | = | 2825.53 | nM | 5.55 |
| CHEMBL1200809 | AZELASTINE HYDROCHLORIDE | Ki | = | 4230.58 | nM | 5.37 |
| CHEMBL4202825 | — | Ki | = | 6674.21 | nM | 5.18 |
| CHEMBL403225 | — | Ki | = | 8406.21 | nM | 5.08 |