Assay Detail
Binding
CHEMBL5442196
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: ADRA2C - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 36 | 6.47 | 8.07 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL300555 | SPIROXATRINE | 2.0 | 1 | 8.07 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | — | 1 | 7.89 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | 4.0 | 1 | 7.70 |
| CHEMBL2106195 | DEXMEDETOMIDINE HYDROCHLORIDE | 4.0 | 1 | 7.41 |
| CHEMBL5076314 | — | — | 1 | 7.40 |
| CHEMBL543113 | — | — | 1 | 7.35 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 7.22 |
| CHEMBL1256876 | — | — | 1 | 7.14 |
| CHEMBL540612 | — | — | 1 | 7.08 |
| CHEMBL1474387 | — | — | 1 | 6.65 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | 4.0 | 1 | 6.63 |
| CHEMBL405355 | NIGULDIPINE | 2.0 | 1 | 6.49 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | 4.0 | 1 | 6.45 |
| CHEMBL24778 | SILODOSIN | 4.0 | 1 | 6.44 |
| CHEMBL267777 | RITANSERIN | 2.0 | 1 | 6.43 |
| CHEMBL5087452 | — | — | 1 | 6.41 |
| CHEMBL1705 | CLONIDINE HYDROCHLORIDE | 4.0 | 1 | 6.37 |
| CHEMBL103769 | — | — | 1 | 6.27 |
| CHEMBL2062257 | — | — | 1 | 6.24 |
| CHEMBL59898 | — | — | 1 | 6.23 |
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | 4.0 | 1 | 6.21 |
| CHEMBL1256998 | — | — | 1 | 6.17 |
| CHEMBL5089005 | — | — | 1 | 6.15 |
| CHEMBL79834 | SPIRAMIDE | 2.0 | 1 | 6.12 |
| CHEMBL1489369 | — | — | 1 | 6.10 |
| CHEMBL1766103 | — | — | 1 | 6.07 |
| CHEMBL83894 | FANANSERIN | 2.0 | 1 | 5.99 |
| CHEMBL679 | EPINEPHRINE | 4.0 | 1 | 5.94 |
| CHEMBL2018875 | — | — | 1 | 5.90 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | 4.0 | 1 | 5.86 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL300555 | SPIROXATRINE | Ki | = | 8.553 | nM | 8.07 |
| CHEMBL1257131 | RAUWOLSCINE HYDROCHLORIDE | Ki | = | 13.01 | nM | 7.89 |
| CHEMBL1200517 | DIHYDROERGOTAMINE MESYLATE | Ki | = | 19.76 | nM | 7.70 |
| CHEMBL2106195 | DEXMEDETOMIDINE HYDROCHLORIDE | Ki | = | 39.39 | nM | 7.41 |
| CHEMBL5076314 | — | Ki | = | 39.91 | nM | 7.40 |
| CHEMBL543113 | — | Ki | = | 44.87 | nM | 7.35 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 59.87 | nM | 7.22 |
| CHEMBL1256876 | — | Ki | = | 71.73 | nM | 7.14 |
| CHEMBL540612 | — | Ki | = | 83.62 | nM | 7.08 |
| CHEMBL1474387 | — | Ki | = | 222.2 | nM | 6.65 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | Ki | = | 234.34 | nM | 6.63 |
| CHEMBL405355 | NIGULDIPINE | Ki | = | 322.11 | nM | 6.49 |
| CHEMBL1200492 | NEFAZODONE HYDROCHLORIDE | Ki | = | 358.92 | nM | 6.45 |
| CHEMBL24778 | SILODOSIN | Ki | = | 360.04 | nM | 6.44 |
| CHEMBL267777 | RITANSERIN | Ki | = | 375.1 | nM | 6.43 |
| CHEMBL5087452 | — | Ki | = | 385.65 | nM | 6.41 |
| CHEMBL1705 | CLONIDINE HYDROCHLORIDE | Ki | = | 431.96 | nM | 6.37 |
| CHEMBL103769 | — | Ki | = | 536.41 | nM | 6.27 |
| CHEMBL2062257 | — | Ki | = | 574.51 | nM | 6.24 |
| CHEMBL59898 | — | Ki | = | 585.19 | nM | 6.23 |
| CHEMBL1558 | PRAZOSIN HYDROCHLORIDE | Ki | = | 617.94 | nM | 6.21 |
| CHEMBL1256998 | — | Ki | = | 673.49 | nM | 6.17 |
| CHEMBL5089005 | — | Ki | = | 704.13 | nM | 6.15 |
| CHEMBL79834 | SPIRAMIDE | Ki | = | 763.6 | nM | 6.12 |
| CHEMBL1489369 | — | Ki | = | 800.94 | nM | 6.10 |
| CHEMBL1766103 | — | Ki | = | 845.67 | nM | 6.07 |
| CHEMBL83894 | FANANSERIN | Ki | = | 1026.13 | nM | 5.99 |
| CHEMBL679 | EPINEPHRINE | Ki | = | 1150.67 | nM | 5.94 |
| CHEMBL2018875 | — | Ki | = | 1259.65 | nM | 5.90 |
| CHEMBL1615374 | VILAZODONE HYDROCHLORIDE | Ki | = | 1378.32 | nM | 5.86 |
| CHEMBL1200776 | CINACALCET HYDROCHLORIDE | Ki | = | 1565.67 | nM | 5.80 |
| CHEMBL2107572 | TECALCET HYDROCHLORIDE | Ki | = | 1581.25 | nM | 5.80 |
| CHEMBL3342358 | — | Ki | = | 1718.3 | nM | 5.76 |
| CHEMBL1256844 | — | Ki | = | 1740.8 | nM | 5.76 |
| CHEMBL18442 | PLERIXAFOR | Ki | = | 1776.03 | nM | 5.75 |
| CHEMBL589390 | LATREPIRDINE | Ki | = | 2924.83 | nM | 5.53 |