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Assay Detail

CHEMBL5442198

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
PDSP Secondary Binding target: DRD3 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
56
Total Activities
56
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Category PDSP assay
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(3) dopamine receptor (CHEMBL234)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

EUbOPEN Chemogenomics Library - PDSP Secondary Binding
EUbOPEN
(2024)
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 56 6.38 9.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2024517 CARIPRAZINE HYDROCHLORIDE 4.0 1 9.01
CHEMBL540612 1 8.65
CHEMBL300555 SPIROXATRINE 2.0 1 8.11
CHEMBL2105760 BREXPIPRAZOLE 4.0 1 7.98
CHEMBL79834 SPIRAMIDE 2.0 1 7.49
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE 4.0 1 7.47
CHEMBL267777 RITANSERIN 2.0 1 7.45
CHEMBL24778 SILODOSIN 4.0 1 7.14
CHEMBL543113 1 6.94
CHEMBL1615374 VILAZODONE HYDROCHLORIDE 4.0 1 6.88
CHEMBL445102 1 6.69
CHEMBL2440454 1 6.57
CHEMBL4743049 1 6.55
CHEMBL542139 1 6.50
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE 4.0 1 6.42
CHEMBL1200776 CINACALCET HYDROCHLORIDE 4.0 1 6.41
CHEMBL1766103 1 6.38
CHEMBL405355 NIGULDIPINE 2.0 1 6.37
CHEMBL1516474 TEGASEROD MALEATE 4.0 1 6.37
CHEMBL5087452 1 6.35
CHEMBL589390 LATREPIRDINE 3.0 1 6.34
CHEMBL2048820 1 6.32
CHEMBL1836317 1 6.30
CHEMBL1256876 1 6.26
CHEMBL1256844 1 6.25
CHEMBL180465 1 6.25
CHEMBL218071 1 6.24
CHEMBL256470 1 6.24
CHEMBL3919973 1 6.23
CHEMBL1200809 AZELASTINE HYDROCHLORIDE 4.0 1 6.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2024517 CARIPRAZINE HYDROCHLORIDE Ki = 0.9749 nM 9.01
CHEMBL540612 Ki = 2.213 nM 8.65
CHEMBL300555 SPIROXATRINE Ki = 7.739 nM 8.11
CHEMBL2105760 BREXPIPRAZOLE Ki = 10.42 nM 7.98
CHEMBL79834 SPIRAMIDE Ki = 32.76 nM 7.49
CHEMBL1200517 DIHYDROERGOTAMINE MESYLATE Ki = 33.96 nM 7.47
CHEMBL267777 RITANSERIN Ki = 35.54 nM 7.45
CHEMBL24778 SILODOSIN Ki = 71.55 nM 7.14
CHEMBL543113 Ki = 115.8 nM 6.94
CHEMBL1615374 VILAZODONE HYDROCHLORIDE Ki = 133.28 nM 6.88
CHEMBL445102 Ki = 205.04 nM 6.69
CHEMBL2440454 Ki = 269.85 nM 6.57
CHEMBL4743049 Ki = 284.08 nM 6.55
CHEMBL542139 Ki = 314.59 nM 6.50
CHEMBL1200492 NEFAZODONE HYDROCHLORIDE Ki = 380.19 nM 6.42
CHEMBL1200776 CINACALCET HYDROCHLORIDE Ki = 390.88 nM 6.41
CHEMBL1766103 Ki = 415.96 nM 6.38
CHEMBL405355 NIGULDIPINE Ki = 424.72 nM 6.37
CHEMBL1516474 TEGASEROD MALEATE Ki = 426.48 nM 6.37
CHEMBL5087452 Ki = 445.35 nM 6.35
CHEMBL589390 LATREPIRDINE Ki = 461.42 nM 6.34
CHEMBL2048820 Ki = 480.01 nM 6.32
CHEMBL1836317 Ki = 498.26 nM 6.30
CHEMBL1256876 Ki = 545.01 nM 6.26
CHEMBL180465 Ki = 558.6 nM 6.25
CHEMBL1256844 Ki = 568.2 nM 6.25
CHEMBL218071 Ki = 582.44 nM 6.24
CHEMBL256470 Ki = 580.7 nM 6.24
CHEMBL3919973 Ki = 590.54 nM 6.23
CHEMBL1200809 AZELASTINE HYDROCHLORIDE Ki = 636.36 nM 6.20
CHEMBL103769 Ki = 641.14 nM 6.19
CHEMBL1256998 Ki = 668.88 nM 6.17
CHEMBL1633 KETOTIFEN FUMARATE Ki = 717.13 nM 6.14
CHEMBL214957 Ki = 767.89 nM 6.12
CHEMBL2107572 TECALCET HYDROCHLORIDE Ki = 874.58 nM 6.06
CHEMBL360648 Ki = 912.36 nM 6.04
CHEMBL2103873 MACITENTAN Ki = 1004.5 nM 6.00
CHEMBL500 PINDOLOL Ki = 994.49 nM 6.00
CHEMBL3342358 Ki = 1018.95 nM 5.99
CHEMBL83894 FANANSERIN Ki = 1022.94 nM 5.99
CHEMBL2386353 Ki = 1097.11 nM 5.96
CHEMBL426559 LAROPIPRANT Ki = 1122.15 nM 5.95
CHEMBL3086703 Ki = 1111.61 nM 5.95
CHEMBL273083 Ki = 1189.78 nM 5.92
CHEMBL1263 SALMETEROL Ki = 1260.37 nM 5.90
CHEMBL1095777 INDACATEROL Ki = 1339.37 nM 5.87
CHEMBL1672558 Ki = 1417.53 nM 5.85
CHEMBL21283 Ki = 1612.13 nM 5.79
CHEMBL1235246 Ki = 1763.87 nM 5.75
CHEMBL49302 Ki = 1958.39 nM 5.71