Assay Detail
Binding
CHEMBL5442213
Review assay metadata, readout intent, target linkage, and publication context from the same page.
PDSP Secondary Binding target: ADRB1 - Compounds are tested at 10 uM concentration, plate are incubated at room temperature in the dark for 90 minutes. Reaction are stopped by vacuum filtration onto 0.3% polyethyleneimine soaked filter mats using Filtermate harvester. Scintillation cocktail is then melted onto microwave-dried filters on a hot plate and the radio activity is counted in Microbia counter. Compounds showing a minimum of 50% inhibition at 10 uM concentration are carried forward in this secondary binding assay to determine equilibrium binding affinity.
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Category | PDSP assay |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
EUbOPEN Chemogenomics Library - PDSP Secondary Binding
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 13 | 6.28 | 8.22 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL499 | TIMOLOL | 4.0 | 1 | 8.22 |
| CHEMBL1691 | BETAXOLOL HYDROCHLORIDE | 4.0 | 1 | 7.69 |
| CHEMBL1374751 | CIMATEROL | 2.0 | 1 | 6.79 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | 4.0 | 1 | 6.65 |
| CHEMBL5087452 | — | — | 1 | 6.38 |
| CHEMBL2105760 | BREXPIPRAZOLE | 4.0 | 1 | 6.23 |
| CHEMBL1263 | SALMETEROL | 4.0 | 1 | 6.16 |
| CHEMBL4743049 | — | — | 1 | 5.69 |
| CHEMBL79834 | SPIRAMIDE | 2.0 | 1 | 5.50 |
| CHEMBL1516474 | TEGASEROD MALEATE | 4.0 | 1 | 5.40 |
| CHEMBL542139 | — | — | 1 | 5.35 |
| CHEMBL519240 | — | — | 1 | 5.33 |
| CHEMBL3341861 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL499 | TIMOLOL | Ki | = | 6.016 | nM | 8.22 |
| CHEMBL1691 | BETAXOLOL HYDROCHLORIDE | Ki | = | 20.57 | nM | 7.69 |
| CHEMBL1374751 | CIMATEROL | Ki | = | 161.49 | nM | 6.79 |
| CHEMBL2024517 | CARIPRAZINE HYDROCHLORIDE | Ki | = | 222.08 | nM | 6.65 |
| CHEMBL5087452 | — | Ki | = | 413.9 | nM | 6.38 |
| CHEMBL2105760 | BREXPIPRAZOLE | Ki | = | 589.86 | nM | 6.23 |
| CHEMBL1263 | SALMETEROL | Ki | = | 694.46 | nM | 6.16 |
| CHEMBL4743049 | — | Ki | = | 2028.15 | nM | 5.69 |
| CHEMBL79834 | SPIRAMIDE | Ki | = | 3131.48 | nM | 5.50 |
| CHEMBL1516474 | TEGASEROD MALEATE | Ki | = | 3990.25 | nM | 5.40 |
| CHEMBL542139 | — | Ki | = | 4462.72 | nM | 5.35 |
| CHEMBL519240 | — | Ki | = | 4622.75 | nM | 5.33 |
| CHEMBL3341861 | — | Ki | > | 10000.0 | nM | - |