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Assay Detail

CHEMBL5501446

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 10 mins by DTNB based Ellman's method
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Equus caballus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL5763)
Type SINGLE PROTEIN
Organism Equus caballus

Publication

Novel 6-alkyl-bridged 4-arylalkylpiperazin-1-yl derivatives of azepino[4,3-b]indol-1(2H)-one as potent BChE-selective inhibitors showing protective effects against neurodegenerative insults.
Samarelli F, Purgatorio R, Lopopolo G, Deruvo C, Catto M, Andresini M, Carrieri A, Nicolotti O, De Palma A, Miniero DV, de Candia M, Altomare CD.
Eur J Med Chem (2024) 270 : 116353 -116353
PubMed 38579622 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 5.92 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL95 TACRINE 4.0 1 7.52
CHEMBL5557312 1 7.19
CHEMBL5558492 1 6.79
CHEMBL5555131 1 6.40
CHEMBL5556474 1 6.37
CHEMBL5532251 1 6.33
CHEMBL5557952 1 6.28
CHEMBL5517704 1 6.14
CHEMBL5532599 1 6.12
CHEMBL5555332 1 6.04
CHEMBL5542859 1 6.00
CHEMBL5556994 1 5.75
CHEMBL5559422 1 5.73
CHEMBL5505916 1 5.72
CHEMBL5557601 1 5.68
CHEMBL502 DONEPEZIL 4.0 1 5.64
CHEMBL5532253 1 5.58
CHEMBL5532065 1 5.31
CHEMBL5517644 1 5.26
CHEMBL5512595 1 5.24
CHEMBL5542336 1 5.18
CHEMBL5532475 1 5.00
CHEMBL5512467 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL95 TACRINE IC50 = 30.0 nM 7.52
CHEMBL5557312 IC50 = 65.0 nM 7.19
CHEMBL5558492 IC50 = 163.0 nM 6.79
CHEMBL5555131 IC50 = 396.0 nM 6.40
CHEMBL5556474 IC50 = 430.0 nM 6.37
CHEMBL5532251 IC50 = 466.0 nM 6.33
CHEMBL5557952 IC50 = 528.0 nM 6.28
CHEMBL5517704 IC50 = 717.0 nM 6.14
CHEMBL5532599 IC50 = 765.0 nM 6.12
CHEMBL5555332 IC50 = 906.0 nM 6.04
CHEMBL5542859 IC50 = 1000.0 nM 6.00
CHEMBL5556994 IC50 = 1780.0 nM 5.75
CHEMBL5559422 IC50 = 1860.0 nM 5.73
CHEMBL5505916 IC50 = 1920.0 nM 5.72
CHEMBL5557601 IC50 = 2100.0 nM 5.68
CHEMBL502 DONEPEZIL IC50 = 2300.0 nM 5.64
CHEMBL5532253 IC50 = 2640.0 nM 5.58
CHEMBL5532065 IC50 = 4920.0 nM 5.31
CHEMBL5517644 IC50 = 5520.0 nM 5.26
CHEMBL5512595 IC50 = 5790.0 nM 5.24
CHEMBL5542336 IC50 = 6590.0 nM 5.18
CHEMBL5532475 IC50 = 10000.0 nM 5.00
CHEMBL5512467 IC50 = 10000.0 nM 5.00