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Assay Detail

CHEMBL5503899

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at M3 receptor in male albino Dunkin-Hartley guinea pig trachea assessed as inhibition of carbachol-induced smooth muscle contraction in presence of propranolol
28
Total Activities
27
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Tissue Trachea
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M3 (CHEMBL5498)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Discovery, Multiparametric Optimization, and Solid-State Driven Identification of CHF-6550, a Novel Soft Dual Pharmacology Muscarinic Antagonist and β<sub>2</sub> Agonist (MABA) for the Inhaled Treatment of Respiratory Diseases.
Carzaniga L, Linney ID, Rizzi A, Schmidt W, Knight CK, Mileo V, Amadei F, Pastore F, Miglietta D, Cesari N, Riccardi B, Mazzucato R, Ghidini E, Blackaby WP, Patacchini R, Battipaglia L, Villetti G, Puccini P, Catinella S, Civelli M, Rancati F.
J Med Chem (2024) 67 : 9816 -9841
PubMed 38857426 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 9.03 9.90
pIC50 11 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5557828 1 9.90
CHEMBL5550055 1 9.70
CHEMBL5172865 1 9.70
CHEMBL5558368 1 9.70
CHEMBL5555665 1 9.40
CHEMBL5532029 1 9.20
CHEMBL5517637 1 9.10
CHEMBL5505816 2 9.10
CHEMBL5556228 1 9.10
CHEMBL5542735 1 9.00
CHEMBL6067691 1 8.70
CHEMBL5505925 1 8.60
CHEMBL5527960 1 8.50
CHEMBL5512435 1 8.50
CHEMBL5555744 1 8.30
CHEMBL6067687 1 8.00
CHEMBL5523458 1 -
CHEMBL5556756 1 -
CHEMBL5542534 1 -
CHEMBL5558093 1 -
CHEMBL5557651 1 -
CHEMBL5542670 1 -
CHEMBL5556694 1 -
CHEMBL5542602 1 -
CHEMBL5527849 1 -
CHEMBL5523340 1 -
CHEMBL5556153 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5557828 IC50 = 0.1259 nM 9.90
CHEMBL5550055 IC50 = 0.1995 nM 9.70
CHEMBL5172865 IC50 = 0.1995 nM 9.70
CHEMBL5558368 IC50 = 0.1995 nM 9.70
CHEMBL5555665 IC50 = 0.3981 nM 9.40
CHEMBL5532029 IC50 = 0.631 nM 9.20
CHEMBL5517637 IC50 = 0.7943 nM 9.10
CHEMBL5505816 IC50 = 0.7943 nM 9.10
CHEMBL5556228 IC50 = 0.7943 nM 9.10
CHEMBL5505816 IC50 = 1.0 nM 9.00
CHEMBL5542735 IC50 = 1.0 nM 9.00
CHEMBL6067691 IC50 = 1.995 nM 8.70
CHEMBL5505925 IC50 = 2.512 nM 8.60
CHEMBL5527960 IC50 = 3.162 nM 8.50
CHEMBL5512435 IC50 = 3.162 nM 8.50
CHEMBL5555744 IC50 = 5.012 nM 8.30
CHEMBL6067687 IC50 = 10.0 nM 8.00
CHEMBL5523458 pIC50 - -
CHEMBL5556756 pIC50 - -
CHEMBL5542534 pIC50 - -
CHEMBL5558093 pIC50 - -
CHEMBL5557651 pIC50 - -
CHEMBL5542670 pIC50 - -
CHEMBL5556694 pIC50 - -
CHEMBL5542602 pIC50 - -
CHEMBL5527849 pIC50 - -
CHEMBL5523340 pIC50 - -
CHEMBL5556153 pIC50 - -