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Assay Detail

CHEMBL5507799

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged recombinant human AAK1 (1 to 510 residues) expressed in insect cells using Aha-KEEQSQITSQVTGQIGWR-NH2 as substrate incubated for 1 hr in presence of ATP by ADP-Glo assay
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

AP2-associated protein kinase 1 (CHEMBL3830)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and biological evaluation of novel 1,2,4a,5-tetrahydro-4H-benzo[b][1,4]oxazino[4,3-d][1,4]oxazine-based AAK1 inhibitors with anti-viral property against SARS-CoV-2.
Mao ND, Xu Y, Che H, Yao X, Gao Y, Wang C, Deng H, Hui Z, Zhang H, Ye XY.
Eur J Med Chem (2024) 268 : 116232 -116232
PubMed 38377825 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.22 8.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4439128 1 8.12
CHEMBL5557266 1 8.03
CHEMBL5564665 1 7.95
CHEMBL5560434 1 7.88
CHEMBL5542596 1 7.81
CHEMBL5555032 1 7.45
CHEMBL5559910 1 7.35
CHEMBL5560369 1 7.32
CHEMBL5557141 1 7.30
CHEMBL5561202 1 7.27
CHEMBL516312 1 7.24
CHEMBL5556790 1 7.22
CHEMBL5561763 1 7.13
CHEMBL535 SUNITINIB 4.0 1 7.11
CHEMBL5566377 1 7.09
CHEMBL5564289 1 7.01
CHEMBL5561927 1 6.98
CHEMBL5566931 1 6.93
CHEMBL5542834 1 6.81
CHEMBL5556067 1 6.81
CHEMBL5517717 1 6.80
CHEMBL5560803 1 6.65
CHEMBL4452939 1 6.53
CHEMBL5518125 1 6.39
CHEMBL5567875 1 -
CHEMBL5517817 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4439128 IC50 = 7.57 nM 8.12
CHEMBL5557266 IC50 = 9.38 nM 8.03
CHEMBL5564665 IC50 = 11.19 nM 7.95
CHEMBL5560434 IC50 = 13.19 nM 7.88
CHEMBL5542596 IC50 = 15.33 nM 7.81
CHEMBL5555032 IC50 = 35.8 nM 7.45
CHEMBL5559910 IC50 = 44.4 nM 7.35
CHEMBL5560369 IC50 = 48.41 nM 7.32
CHEMBL5557141 IC50 = 50.1 nM 7.30
CHEMBL5561202 IC50 = 53.62 nM 7.27
CHEMBL516312 IC50 = 57.81 nM 7.24
CHEMBL5556790 IC50 = 59.67 nM 7.22
CHEMBL5561763 IC50 = 74.68 nM 7.13
CHEMBL535 SUNITINIB IC50 = 76.86 nM 7.11
CHEMBL5566377 IC50 = 81.68 nM 7.09
CHEMBL5564289 IC50 = 97.2 nM 7.01
CHEMBL5561927 IC50 = 103.96 nM 6.98
CHEMBL5566931 IC50 = 118.68 nM 6.93
CHEMBL5542834 IC50 = 155.33 nM 6.81
CHEMBL5556067 IC50 = 154.77 nM 6.81
CHEMBL5517717 IC50 = 160.0 nM 6.80
CHEMBL5560803 IC50 = 225.14 nM 6.65
CHEMBL4452939 IC50 = 295.6 nM 6.53
CHEMBL5518125 IC50 = 406.03 nM 6.39
CHEMBL5567875 IC50 > 500.0 nM -
CHEMBL5517817 IC50 > 500.0 nM -