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Assay Detail

CHEMBL5531340

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at GluN1/GluN2B receptor (unknown origin) expressed in HEK293 cells assessed as inhibition of receptor-mediated current by patch clamp assay
8
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2B (CHEMBL1907603)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Design, Synthesis, and Biological Evaluation of <i>Pierardine</i> Derivatives as Novel Brain-Penetrant and <i>In Vivo</i> Potent NMDAR-GluN2B Antagonists for Ischemic Stroke Treatment.
Lin G, Xu Q, Li J, Chu Z, Ma X, Zhu Q, Zhao Y, Mo J, Ye W, Shao L, Fang T, He M, Yue S, Dai M.
J Med Chem (2024) 67 : 3358 -3384
PubMed 38413367 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.90 7.13
Activity 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5570052 1 7.13
CHEMBL305187 IFENPRODIL 2.0 1 6.89
CHEMBL5563730 2 6.82
CHEMBL5569595 2 6.74
CHEMBL5208814 1 -
CHEMBL5567588 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5570052 IC50 = 74.01 nM 7.13
CHEMBL305187 IFENPRODIL IC50 = 127.8 nM 6.89
CHEMBL5563730 IC50 = 152.66 nM 6.82
CHEMBL5569595 IC50 = 183.04 nM 6.74
CHEMBL5208814 IC50 > 10000.0 nM -
CHEMBL5563730 Activity - -
CHEMBL5569595 Activity - -
CHEMBL5567588 IC50 > 10.0 nM -