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Assay Detail

CHEMBL5537008

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine TNAP using CDP-star as substrate preincubated with enzyme for 3 to 5 mins followed by substrate addition and measured after 15 mins by luminescence based analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alkaline phosphatase, tissue-nonspecific isozyme (CHEMBL2406894)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Unveiling sultam in drug discovery: spotlight on the underexplored scaffold.
Chong YK, Ong YS, Yeong KY.
RSC Med Chem (2024) 15 : 1798 -1827
PubMed 38911171 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.24 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5595268 1 6.96
CHEMBL5562714 1 6.72
CHEMBL5565700 1 6.70
CHEMBL5573727 1 6.57
CHEMBL5591314 1 6.57
CHEMBL5562682 1 6.52
CHEMBL5571035 1 6.51
CHEMBL5591499 1 6.38
CHEMBL5562695 1 6.36
CHEMBL5583594 1 6.34
CHEMBL5569727 1 6.30
CHEMBL5591327 1 5.92
CHEMBL5566772 1 5.84
CHEMBL5573339 1 5.18
CHEMBL1454 LEVAMISOLE 4.0 1 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5595268 IC50 = 110.0 nM 6.96
CHEMBL5562714 IC50 = 190.0 nM 6.72
CHEMBL5565700 IC50 = 200.0 nM 6.70
CHEMBL5573727 IC50 = 270.0 nM 6.57
CHEMBL5591314 IC50 = 270.0 nM 6.57
CHEMBL5562682 IC50 = 300.0 nM 6.52
CHEMBL5571035 IC50 = 310.0 nM 6.51
CHEMBL5591499 IC50 = 420.0 nM 6.38
CHEMBL5562695 IC50 = 440.0 nM 6.36
CHEMBL5583594 IC50 = 460.0 nM 6.34
CHEMBL5569727 IC50 = 500.0 nM 6.30
CHEMBL5591327 IC50 = 1190.0 nM 5.92
CHEMBL5566772 IC50 = 1450.0 nM 5.84
CHEMBL5573339 IC50 = 6630.0 nM 5.18
CHEMBL1454 LEVAMISOLE IC50 = 19210.0 nM 4.72