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Assay Detail

CHEMBL5541685

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABL (unknown origin) using FAM-P22 peptide as substrate preincubated with compound for 10 mins followed by substrate addition and ATP measured after 60 mins by fluorescence-based microfluidic mobility shift assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase ABL1 (CHEMBL1862)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of Piperazinyl-Difluoro-indene Derivatives Containing Pyridyl Groups as Potent FGFR Inhibitors against FGFR Mutant Tumor: Design, Synthesis, and Biological Evaluation.
Ding W, Yan L, Sheng L, Chen S, Li Y, Cheng S, Luo L, Huang H, Shao H, Zhang D.
J Med Chem (2024) 67 : 2941 -2962
PubMed 38294952 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.37 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5571836 1 7.75
CHEMBL5570514 1 7.68
CHEMBL388978 STAUROSPORINE 1 6.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5571836 IC50 = 17.7 nM 7.75
CHEMBL5570514 IC50 = 20.9 nM 7.68
CHEMBL388978 STAUROSPORINE IC50 = 208.3 nM 6.68