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Assay Detail

CHEMBL5547428

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Functional
Agonist activity at human 5-HT2A receptor expressed in HEK293 cells assessed as IP2 production measured after 30 mins incubation by HTRF assay
22
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Structure-Activity Relationships of 2,5-Dimethoxy-4-Substituted Phenethylamines and the Discovery of CYB210010: A Potent, Orally Bioavailable and Long-Acting Serotonin 5-HT<sub>2</sub> Receptor Agonist.
Varty GB, Canal CE, Mueller TA, Hartsel JA, Tyagi R, Avery K, Morgan ME, Reichelt AC, Pathare P, Stang E, Palfreyman MG, Nivorozhkin A.
J Med Chem (2024) 67 : 6144 -6188
PubMed 38593423 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 22 7.88 9.18

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5592769 2 9.18
CHEMBL5591197 2 8.74
CHEMBL5566821 2 8.45
CHEMBL5575032 1 8.38
CHEMBL5566894 2 8.27
CHEMBL5566866 2 8.21
CHEMBL5563475 2 7.39
CHEMBL5571221 2 7.32
CHEMBL5569144 1 7.05
CHEMBL127285 1 6.82
CHEMBL5592265 1 6.28
CHEMBL5565571 1 6.07
CHEMBL5592656 1 -
CHEMBL5590167 1 -
CHEMBL5566311 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5592769 EC50 = 0.6607 nM 9.18
CHEMBL5592769 EC50 = 0.67 nM 9.17
CHEMBL5591197 EC50 = 1.8 nM 8.74
CHEMBL5591197 EC50 = 1.995 nM 8.70
CHEMBL5566821 EC50 = 3.548 nM 8.45
CHEMBL5566821 EC50 = 3.6 nM 8.44
CHEMBL5575032 EC50 = 4.2 nM 8.38
CHEMBL5566894 EC50 = 5.4 nM 8.27
CHEMBL5566894 EC50 = 5.495 nM 8.26
CHEMBL5566866 EC50 = 6.1 nM 8.21
CHEMBL5566866 EC50 = 6.166 nM 8.21
CHEMBL5563475 EC50 = 41.0 nM 7.39
CHEMBL5563475 EC50 = 41.69 nM 7.38
CHEMBL5571221 EC50 = 47.86 nM 7.32
CHEMBL5571221 EC50 = 48.0 nM 7.32
CHEMBL5569144 EC50 = 89.0 nM 7.05
CHEMBL127285 EC50 = 150.0 nM 6.82
CHEMBL5592265 EC50 = 530.0 nM 6.28
CHEMBL5565571 EC50 = 850.0 nM 6.07
CHEMBL5566311 EC50 - -
CHEMBL5590167 EC50 - -
CHEMBL5592656 EC50 - -