Assay Detail
Binding
CHEMBL5548356
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Positive allosteric modulation of EAAT1 (unknown origin) transfected in African green monkey COS-7 cells assessed as increase in glutamate uptake preincubated for 10 mins followed by 3-H-L-glutamate addition and measured after 10 mins by scintillation counter analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | COS-7 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and Structure-Activity Relationships for Glutamate Transporter Allosteric Modulators.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 10 | 8.54 | 10.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5571220 | — | — | 1 | 10.70 |
| CHEMBL5563875 | — | — | 1 | 9.22 |
| CHEMBL5593916 | — | — | 1 | 8.80 |
| CHEMBL5564905 | — | — | 1 | 8.70 |
| CHEMBL5571181 | — | — | 1 | 8.65 |
| CHEMBL5594077 | — | — | 1 | 8.56 |
| CHEMBL5590998 | — | — | 1 | 8.40 |
| CHEMBL5573416 | — | — | 1 | 8.28 |
| CHEMBL5583680 | — | — | 1 | 7.24 |
| CHEMBL1393473 | — | — | 1 | 6.88 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5571220 | — | EC50 | = | 0.02 | nM | 10.70 |
| CHEMBL5563875 | — | EC50 | = | 0.6 | nM | 9.22 |
| CHEMBL5593916 | — | EC50 | = | 1.6 | nM | 8.80 |
| CHEMBL5564905 | — | EC50 | = | 2.0 | nM | 8.70 |
| CHEMBL5571181 | — | EC50 | = | 2.24 | nM | 8.65 |
| CHEMBL5594077 | — | EC50 | = | 2.75 | nM | 8.56 |
| CHEMBL5590998 | — | EC50 | = | 4.0 | nM | 8.40 |
| CHEMBL5573416 | — | EC50 | = | 5.3 | nM | 8.28 |
| CHEMBL5583680 | — | EC50 | = | 58.0 | nM | 7.24 |
| CHEMBL1393473 | — | EC50 | = | 132.0 | nM | 6.88 |