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Assay Detail

CHEMBL5552296

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human N-terminal his tagged Hsp90beta (1 to 239 residues) expressed in Escherichia coli (DE3) strain assessed as dissociation constant by Fluorescent thermal shift assay
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Heat shock protein HSP 90-beta (CHEMBL4303)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Comprehensive analysis of resorcinyl-imidazole Hsp90 inhibitor design.
Gedgaudas M, Kaziukonytė P, Kairys V, Mickevičiūtė A, Zubrienė A, Brukštus A, Matulis D, Kazlauskas E.
Eur J Med Chem (2024) 273 : 116505 -116505
PubMed 38788300 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 32 5.67 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5574581 1 8.70
CHEMBL5569309 1 8.40
CHEMBL5564393 1 8.30
CHEMBL5568823 1 7.96
CHEMBL5562891 1 6.36
CHEMBL5570148 1 5.77
CHEMBL5563338 1 5.75
CHEMBL5574005 1 5.47
CHEMBL5565341 1 5.33
CHEMBL5567226 1 5.27
CHEMBL5573532 1 5.26
CHEMBL5571438 1 5.26
CHEMBL5573792 1 5.22
CHEMBL5562627 1 5.17
CHEMBL5574513 1 5.16
CHEMBL5566352 1 5.10
CHEMBL5564811 1 5.10
CHEMBL5574697 1 5.02
CHEMBL5572196 1 5.00
CHEMBL5595824 1 4.92
CHEMBL5573452 1 4.77
CHEMBL5571855 1 4.34
CHEMBL5573001 1 4.24
CHEMBL5567036 1 4.10
CHEMBL5570869 1 -
CHEMBL5571440 1 -
CHEMBL5579749 1 -
CHEMBL5569544 1 -
CHEMBL5569716 1 -
CHEMBL5564920 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5574581 Kd = 2.0 nM 8.70
CHEMBL5569309 Kd = 4.0 nM 8.40
CHEMBL5564393 Kd = 5.0 nM 8.30
CHEMBL5568823 Kd = 11.0 nM 7.96
CHEMBL5562891 Kd = 440.0 nM 6.36
CHEMBL5570148 Kd = 1700.0 nM 5.77
CHEMBL5563338 Kd = 1800.0 nM 5.75
CHEMBL5574005 Kd = 3400.0 nM 5.47
CHEMBL5565341 Kd = 4700.0 nM 5.33
CHEMBL5567226 Kd = 5400.0 nM 5.27
CHEMBL5573532 Kd = 5500.0 nM 5.26
CHEMBL5571438 Kd = 5500.0 nM 5.26
CHEMBL5573792 Kd = 6000.0 nM 5.22
CHEMBL5562627 Kd = 6800.0 nM 5.17
CHEMBL5574513 Kd = 6900.0 nM 5.16
CHEMBL5564811 Kd = 7900.0 nM 5.10
CHEMBL5566352 Kd = 7900.0 nM 5.10
CHEMBL5574697 Kd = 9500.0 nM 5.02
CHEMBL5572196 Kd = 10000.0 nM 5.00
CHEMBL5595824 Kd = 12000.0 nM 4.92
CHEMBL5573452 Kd = 17000.0 nM 4.77
CHEMBL5571855 Kd = 46000.0 nM 4.34
CHEMBL5573001 Kd = 57000.0 nM 4.24
CHEMBL5567036 Kd = 79000.0 nM 4.10
CHEMBL5570869 Kd >= 200000.0 nM -
CHEMBL5571440 Kd >= 200000.0 nM -
CHEMBL5579749 Kd >= 200000.0 nM -
CHEMBL5569544 Kd >= 200000.0 nM -
CHEMBL5569716 Kd >= 200000.0 nM -
CHEMBL5564920 Kd >= 200000.0 nM -
CHEMBL5575085 Kd >= 200000.0 nM -
CHEMBL5573512 Kd >= 200000.0 nM -