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Assay Detail

CHEMBL5553826

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human haspin preincubated for 30 mins followed by ATP and ULight-histone H3 peptide/ULight-eIF4E binding protein addition and measured after 2 hrs by LANCE Ultra TR-FRET assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase haspin (CHEMBL1075163)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A Comprehensive <i>In Vitro</i> Characterization of a New Class of Indole-Based Compounds Developed as Selective Haspin Inhibitors.
Vestuto V, Ciaglia T, Musella S, Di Sarno V, Smaldone G, Di Matteo F, Scala MC, Napolitano V, Miranda MR, Amodio G, Novi S, Pepe G, Basilicata MG, Gazzillo E, Pace S, Gomez-Monterrey IM, Sala M, Bifulco G, Tecce MF, Campiglia P, Ostacolo C, Lauro G, Manfra M, Bertamino A.
J Med Chem (2024) 67 : 12711 -12734
PubMed 39038808 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 6.66 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5563528 1 8.52
CHEMBL5178368 1 8.40
CHEMBL5575484 1 8.30
CHEMBL4446681 1 8.22
CHEMBL5566424 1 8.15
CHEMBL5566041 1 7.19
CHEMBL5595605 1 6.98
CHEMBL5572441 1 6.80
CHEMBL5571554 1 6.45
CHEMBL5570156 1 6.42
CHEMBL5565688 1 6.18
CHEMBL5568999 1 6.04
CHEMBL5591651 1 6.01
CHEMBL5574750 1 6.00
CHEMBL5595763 1 5.95
CHEMBL5564624 1 5.94
CHEMBL5593072 1 5.93
CHEMBL5591618 1 5.83
CHEMBL5583809 1 4.94
CHEMBL5564324 1 4.88
CHEMBL5571240 1 -
CHEMBL5564931 1 -
CHEMBL5571306 1 -
CHEMBL5591602 1 -
CHEMBL5574302 1 -
CHEMBL5575275 1 -
CHEMBL5576026 1 -
CHEMBL5563118 1 -
CHEMBL5575835 1 -
CHEMBL5569783 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5563528 IC50 = 3.0 nM 8.52
CHEMBL5178368 IC50 = 4.0 nM 8.40
CHEMBL5575484 IC50 = 5.0 nM 8.30
CHEMBL4446681 IC50 = 6.0 nM 8.22
CHEMBL5566424 IC50 = 7.0 nM 8.15
CHEMBL5566041 IC50 = 65.0 nM 7.19
CHEMBL5595605 IC50 = 105.0 nM 6.98
CHEMBL5572441 IC50 = 160.0 nM 6.80
CHEMBL5571554 IC50 = 353.0 nM 6.45
CHEMBL5570156 IC50 = 380.0 nM 6.42
CHEMBL5565688 IC50 = 666.0 nM 6.18
CHEMBL5568999 IC50 = 922.0 nM 6.04
CHEMBL5591651 IC50 = 975.0 nM 6.01
CHEMBL5574750 IC50 = 1000.0 nM 6.00
CHEMBL5595763 IC50 = 1135.0 nM 5.95
CHEMBL5564624 IC50 = 1148.0 nM 5.94
CHEMBL5593072 IC50 = 1168.0 nM 5.93
CHEMBL5591618 IC50 = 1463.0 nM 5.83
CHEMBL5583809 IC50 = 11590.0 nM 4.94
CHEMBL5564324 IC50 = 13150.0 nM 4.88
CHEMBL5571240 IC50 - -
CHEMBL5564931 IC50 - -
CHEMBL5571306 IC50 - -
CHEMBL5591602 IC50 - -
CHEMBL5574302 IC50 = 0.035 10^-4microM -
CHEMBL5575275 IC50 - -
CHEMBL5576026 IC50 = 0.084 10^-4microM -
CHEMBL5563118 IC50 = 0.002 10^-4microM -
CHEMBL5575835 IC50 = 1.0 10^-5 uM -
CHEMBL5569783 IC50 - -
CHEMBL5567189 IC50 - -