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Assay Detail

CHEMBL5609169

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [35S]TBPS binding to GABA-A receptor in Sprague-Dawley rat brain cortical membrane incubated for 90 mins by liquid scintillation spectrometry
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cortex
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Synthesis and evaluation of neuroactive steroids with novel pharmacophore at C-21 let identify a compound with advantageous PK profile and higher EC<sub>50</sub> and E<sub>max</sub> as PAM on GABAA receptor.
Ma M, Xu H, Ye L, Li C, Zhu H, Jiang W, Wang W, Yang H, Yang Y, Wang Y, Tian J.
Eur J Med Chem (2024) 276 : 116602 -116602
PubMed 38971049 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 8.81 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5612974 1 9.00
CHEMBL5614143 1 9.00
CHEMBL5612574 1 9.00
CHEMBL5612649 1 8.92
CHEMBL4105630 ZURANOLONE 4.0 1 8.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5612974 IC50 = 1.0 nM 9.00
CHEMBL5614143 IC50 = 1.0 nM 9.00
CHEMBL5612574 IC50 = 1.0 nM 9.00
CHEMBL5612649 IC50 = 1.2 nM 8.92
CHEMBL4105630 ZURANOLONE IC50 = 7.0 nM 8.15