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Assay Detail

CHEMBL5621144

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of equine serum BuChE using butyrylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition and measured after 20 mins by DTNB reagent based Ellman's method
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Equus caballus
Tissue Serum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL5763)
Type SINGLE PROTEIN
Organism Equus caballus

Publication

Sulfur-fluoride exchange (SuFEx)-enabled lead discovery of AChE inhibitors by fragment linking strategies.
Zhang Z, Zhang SL, Wu C, Li HH, Zha L, Shi J, Liu X, Qin HL, Tang W.
Eur J Med Chem (2023) 257 : 115502 -115502
PubMed 37224761 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.36 7.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL636 RIVASTIGMINE 4.0 1 7.28
CHEMBL5624584 1 5.48
CHEMBL5630661 1 5.30
CHEMBL5630544 1 5.10
CHEMBL502 DONEPEZIL 4.0 1 5.01
CHEMBL5624445 1 4.97
CHEMBL5630831 1 4.92
CHEMBL5624533 1 4.85
CHEMBL5629983 1 -
CHEMBL5630220 1 -
CHEMBL5625068 1 -
CHEMBL5624473 1 -
CHEMBL5624757 1 -
CHEMBL5630049 1 -
CHEMBL5630704 1 -
CHEMBL5624457 1 -
CHEMBL5630674 1 -
CHEMBL5624597 1 -
CHEMBL5629892 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL636 RIVASTIGMINE IC50 = 52.0 nM 7.28
CHEMBL5624584 IC50 = 3300.0 nM 5.48
CHEMBL5630661 IC50 = 5030.0 nM 5.30
CHEMBL5630544 IC50 = 7980.0 nM 5.10
CHEMBL502 DONEPEZIL IC50 = 9830.0 nM 5.01
CHEMBL5624445 IC50 = 10690.0 nM 4.97
CHEMBL5630831 IC50 = 11970.0 nM 4.92
CHEMBL5624533 IC50 = 14020.0 nM 4.85
CHEMBL5629983 IC50 - -
CHEMBL5630220 IC50 - -
CHEMBL5625068 IC50 - -
CHEMBL5624473 IC50 - -
CHEMBL5624757 IC50 - -
CHEMBL5630049 IC50 - -
CHEMBL5630704 IC50 - -
CHEMBL5624457 IC50 - -
CHEMBL5630674 IC50 - -
CHEMBL5624597 IC50 - -
CHEMBL5629892 IC50 - -