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Assay Detail

CHEMBL5657595

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP2C9 in human pooled liver microsomes using diclofenac as substrate measured after 10 to 20 mins by LC-MS/MS analysis
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 2C9 (CHEMBL3397)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Freezing the Bioactive Conformation to Boost Potency: The Identification of BAY 85-8501, a Selective and Potent Inhibitor of Human Neutrophil Elastase for Pulmonary Diseases.
von Nussbaum F, Li VM, Allerheiligen S, Anlauf S, Bärfacker L, Bechem M, Delbeck M, Fitzgerald MF, Gerisch M, Gielen-Haertwig H, Haning H, Karthaus D, Lang D, Lustig K, Meibom D, Mittendorf J, Rosentreter U, Schäfer M, Schäfer S, Schamberger J, Telan LA, Tersteegen A.
ChemMedChem (2015) 10 : 1163 -1173
PubMed 26083237 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.30 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL5661905 1 6.30
CHEMBL5661893 1 6.22
CHEMBL1738716 1 5.70
CHEMBL5661903 1 5.70
CHEMBL5661864 1 5.30
CHEMBL5661879 1 5.16
CHEMBL5661854 1 5.10
CHEMBL3617968 BAY-678 1 4.92
CHEMBL5661856 1 4.75
CHEMBL5661871 1 4.64
CHEMBL3617969 1 4.51
CHEMBL5661868 1 -
CHEMBL5661863 1 -
CHEMBL5661882 1 -
CHEMBL5661928 1 -
CHEMBL5661860 1 -
CHEMBL3617972 1 -
CHEMBL3617973 1 -
CHEMBL5661855 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL5661905 IC50 = 500.0 nM 6.30
CHEMBL5661893 IC50 = 600.0 nM 6.22
CHEMBL1738716 IC50 = 2000.0 nM 5.70
CHEMBL5661903 IC50 = 2000.0 nM 5.70
CHEMBL5661864 IC50 = 5000.0 nM 5.30
CHEMBL5661879 IC50 = 7000.0 nM 5.16
CHEMBL5661854 IC50 = 8000.0 nM 5.10
CHEMBL3617968 BAY-678 IC50 = 12000.0 nM 4.92
CHEMBL5661856 IC50 = 18000.0 nM 4.75
CHEMBL5661871 IC50 = 23000.0 nM 4.64
CHEMBL3617969 IC50 = 31000.0 nM 4.51
CHEMBL5661868 IC50 > 50000.0 nM -
CHEMBL5661863 IC50 > 50000.0 nM -
CHEMBL5661882 IC50 > 50000.0 nM -
CHEMBL5661928 IC50 > 50000.0 nM -
CHEMBL5661860 IC50 > 50000.0 nM -
CHEMBL3617972 IC50 > 50000.0 nM -
CHEMBL3617973 IC50 > 50000.0 nM -
CHEMBL5661855 IC50 > 50000.0 nM -