Assay Detail
Binding
CHEMBL5659516
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of PRMT5 in human Z138 cells assessed as effect on SDMA level incubated for 3 days by ELISA analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
2
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Z138 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Protein arginine N-methyltransferase 5 (CHEMBL1795116) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Activation of the p53-MDM4 regulatory axis defines the anti-tumour response to PRMT5 inhibition through its role in regulating cellular splicing.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 2 | 8.64 | 8.68 |
Assay Parameters
| Parameter | Type | Value | Units | Text |
|---|---|---|---|---|
| EFO_ID | EFO_ID | - | — | MONDO:0004992 |
| EFO_TERM | EFO_TERM | - | — | cancer |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4542380 | — | — | 1 | 8.68 |
| CHEMBL4466233 | PEMRAMETOSTAT | 2.0 | 1 | 8.60 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4542380 | — | EC50 | = | 2.1 | nM | 8.68 |
| CHEMBL4466233 | PEMRAMETOSTAT | EC50 | = | 2.5 | nM | 8.60 |