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Assay Detail

CHEMBL5683280

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of LRRK2 G2019S mutant (unknown origin) using LRRKtide as substrate incubated for 1 hrs in presence of ATP by Adapta TR-FRET assay
22
Total Activities
22
Compounds Tested
1
Activity Types
2
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Structural determinants for ERK5 (MAPK7) and leucine rich repeat kinase 2 activities of benzo[e]pyrimido-[5,4-b]diazepine-6(11H)-ones.
Deng X, Elkins JM, Zhang J, Yang Q, Erazo T, Gomez N, Choi HG, Wang J, Dzamko N, Lee JD, Sim T, Kim N, Alessi DR, Lizcano JM, Knapp S, Gray NS.
Eur J Med Chem (2013) 70 : 758 -767
PubMed 24239623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 7.02 8.40

Assay Parameters

Parameter Type Value Units Text
EFO_ID EFO_ID - MONDO:0004992
EFO_TERM EFO_TERM - cancer

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1673043 1 8.40
CHEMBL2381339 1 8.30
CHEMBL5686992 1 8.30
CHEMBL1673048 1 8.10
CHEMBL1673040 1 7.77
CHEMBL1673039 1 7.58
CHEMBL1673041 1 7.51
CHEMBL1673051 1 7.28
CHEMBL1673046 1 7.23
CHEMBL5687018 1 7.21
CHEMBL1673047 1 7.16
CHEMBL1672987 1 7.04
CHEMBL4632948 1 6.59
CHEMBL2381340 1 6.47
CHEMBL1673052 1 6.44
CHEMBL5686989 1 6.36
CHEMBL1673053 1 6.11
CHEMBL1673057 1 5.97
CHEMBL5687010 1 5.62
CHEMBL1673056 1 5.00
CHEMBL5687020 1 -
CHEMBL5687005 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1673043 IC50 = 4.0 nM 8.40
CHEMBL2381339 IC50 = 5.0 nM 8.30
CHEMBL5686992 IC50 = 5.0 nM 8.30
CHEMBL1673048 IC50 = 8.0 nM 8.10
CHEMBL1673040 IC50 = 17.0 nM 7.77
CHEMBL1673039 IC50 = 26.0 nM 7.58
CHEMBL1673041 IC50 = 31.0 nM 7.51
CHEMBL1673051 IC50 = 52.0 nM 7.28
CHEMBL1673046 IC50 = 59.0 nM 7.23
CHEMBL5687018 IC50 = 61.0 nM 7.21
CHEMBL1673047 IC50 = 70.0 nM 7.16
CHEMBL1672987 IC50 = 91.0 nM 7.04
CHEMBL4632948 IC50 = 256.0 nM 6.59
CHEMBL2381340 IC50 = 339.0 nM 6.47
CHEMBL1673052 IC50 = 360.0 nM 6.44
CHEMBL5686989 IC50 = 439.0 nM 6.36
CHEMBL1673053 IC50 = 781.0 nM 6.11
CHEMBL1673057 IC50 = 1070.0 nM 5.97
CHEMBL5687010 IC50 = 2370.0 nM 5.62
CHEMBL1673056 IC50 = 10000.0 nM 5.00
CHEMBL5687020 IC50 > 493.0 nM -
CHEMBL5687005 IC50 > 10000.0 nM -