Assay Detail
Binding
CHEMBL5734559
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition Assay: The tankyrase-1 or tankyrase-2 enzyme inhibitory activities of the compounds of the present invention were assayed in the following manner by use of a kit (cat. 80573 or 80578) purchased from BPS Bioscience.The 96-well plate provided in BPS Bioscience kit was coated with histone and incubated at 4° C. for 16 hours. Then, the plate was washed four times with PBST (7.5 mM Na2HPO4, 2.5 mM NaH2PO4, 145 mM NaCl, 0.05% Tween 20, pH 7.4), and blocking buffer (provided in BPS Bioscience kit) was added thereto in order to block nonspecific reaction, and was then incubated at 25° C. for 1 hour. After incubation for 1 hour, the plate was washed four times with PBST, and varying concentrations of each of the compounds of the Examples were added to a reaction solution containing tankyrase-1 enzyme (40 ng/well) or tankyrase-2 enzyme (15 ng/well) and an assay mixture, and allowed to react at 25° C. for 1 hour. After 1 hour, each well was washed four times with PBST, and in order to measure the level of ribosylation by PARP enzyme, streptavidin-linked peroxidase (Strep-HRP, 1:50 dilution) was added and allowed to react at 25° C. for 30 minutes. The plate was washed four times with PBST, and then finally an HRP chemiluminescent substrate was added and allowed to react. The level of histone ribosylation formed by each enzyme was quantified using Synergy H4 Hybrid Multi-Mode Microplate Reader (BioTek Instruments, Inc., USA).
171
Total Activities
57
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Tricyclic derivative compound, method for preparing same, and pharmaceutical composition comprising same
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 57 | 8.35 | 9.04 |
| kon | 57 | - | - |
| k_off | 57 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL5772004 | — | — | 3 | 9.04 |
| CHEMBL6022678 | — | — | 3 | 9.02 |
| CHEMBL5853387 | — | — | 3 | 8.90 |
| CHEMBL5756581 | — | — | 3 | 8.88 |
| CHEMBL5968204 | — | — | 3 | 8.88 |
| CHEMBL6054797 | — | — | 3 | 8.86 |
| CHEMBL5095245 | NESUPARIB | 2.0 | 3 | 8.83 |
| CHEMBL5795408 | — | — | 3 | 8.78 |
| CHEMBL5822783 | — | — | 3 | 8.76 |
| CHEMBL5932989 | — | — | 3 | 8.76 |
| CHEMBL5997953 | — | — | 3 | 8.73 |
| CHEMBL6001595 | — | — | 3 | 8.71 |
| CHEMBL6058132 | — | — | 3 | 8.71 |
| CHEMBL5924751 | — | — | 3 | 8.70 |
| CHEMBL6023648 | — | — | 3 | 8.68 |
| CHEMBL5879210 | — | — | 3 | 8.67 |
| CHEMBL5993654 | — | — | 3 | 8.62 |
| CHEMBL5989650 | — | — | 3 | 8.62 |
| CHEMBL5955353 | — | — | 3 | 8.61 |
| CHEMBL5772340 | — | — | 3 | 8.58 |
| CHEMBL5758369 | — | — | 3 | 8.57 |
| CHEMBL5750491 | — | — | 3 | 8.55 |
| CHEMBL5869375 | — | — | 3 | 8.54 |
| CHEMBL5949390 | — | — | 3 | 8.50 |
| CHEMBL5851052 | — | — | 3 | 8.49 |
| CHEMBL5783189 | — | — | 3 | 8.47 |
| CHEMBL5788476 | — | — | 3 | 8.46 |
| CHEMBL5785121 | — | — | 3 | 8.43 |
| CHEMBL6041990 | — | — | 3 | 8.42 |
| CHEMBL5863835 | — | — | 3 | 8.42 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL5772004 | — | IC50 | = | 0.92 | nM | 9.04 |
| CHEMBL6022678 | — | IC50 | = | 0.95 | nM | 9.02 |
| CHEMBL5853387 | — | IC50 | = | 1.26 | nM | 8.90 |
| CHEMBL5756581 | — | IC50 | = | 1.31 | nM | 8.88 |
| CHEMBL5968204 | — | IC50 | = | 1.32 | nM | 8.88 |
| CHEMBL6054797 | — | IC50 | = | 1.38 | nM | 8.86 |
| CHEMBL5095245 | NESUPARIB | IC50 | = | 1.48 | nM | 8.83 |
| CHEMBL5795408 | — | IC50 | = | 1.65 | nM | 8.78 |
| CHEMBL5932989 | — | IC50 | = | 1.75 | nM | 8.76 |
| CHEMBL5822783 | — | IC50 | = | 1.75 | nM | 8.76 |
| CHEMBL5997953 | — | IC50 | = | 1.87 | nM | 8.73 |
| CHEMBL6058132 | — | IC50 | = | 1.97 | nM | 8.71 |
| CHEMBL6001595 | — | IC50 | = | 1.96 | nM | 8.71 |
| CHEMBL5924751 | — | IC50 | = | 1.99 | nM | 8.70 |
| CHEMBL6023648 | — | IC50 | = | 2.1 | nM | 8.68 |
| CHEMBL5879210 | — | IC50 | = | 2.12 | nM | 8.67 |
| CHEMBL5993654 | — | IC50 | = | 2.38 | nM | 8.62 |
| CHEMBL5989650 | — | IC50 | = | 2.37 | nM | 8.62 |
| CHEMBL5955353 | — | IC50 | = | 2.45 | nM | 8.61 |
| CHEMBL5772340 | — | IC50 | = | 2.62 | nM | 8.58 |
| CHEMBL5758369 | — | IC50 | = | 2.7 | nM | 8.57 |
| CHEMBL5750491 | — | IC50 | = | 2.81 | nM | 8.55 |
| CHEMBL5869375 | — | IC50 | = | 2.86 | nM | 8.54 |
| CHEMBL5949390 | — | IC50 | = | 3.18 | nM | 8.50 |
| CHEMBL5851052 | — | IC50 | = | 3.24 | nM | 8.49 |
| CHEMBL5783189 | — | IC50 | = | 3.38 | nM | 8.47 |
| CHEMBL5788476 | — | IC50 | = | 3.49 | nM | 8.46 |
| CHEMBL5785121 | — | IC50 | = | 3.7 | nM | 8.43 |
| CHEMBL5863835 | — | IC50 | = | 3.78 | nM | 8.42 |
| CHEMBL6041990 | — | IC50 | = | 3.8 | nM | 8.42 |
| CHEMBL5902193 | — | IC50 | = | 4.2 | nM | 8.38 |
| CHEMBL5767150 | — | IC50 | = | 4.36 | nM | 8.36 |
| CHEMBL5954322 | — | IC50 | = | 4.71 | nM | 8.33 |
| CHEMBL6064240 | — | IC50 | = | 4.63 | nM | 8.33 |
| CHEMBL6013549 | — | IC50 | = | 4.82 | nM | 8.32 |
| CHEMBL5960080 | — | IC50 | = | 4.96 | nM | 8.30 |
| CHEMBL5813929 | — | IC50 | = | 6.06 | nM | 8.22 |
| CHEMBL5958241 | — | IC50 | = | 6.21 | nM | 8.21 |
| CHEMBL5921357 | — | IC50 | = | 6.47 | nM | 8.19 |
| CHEMBL6009619 | — | IC50 | = | 6.59 | nM | 8.18 |
| CHEMBL5762886 | — | IC50 | = | 6.99 | nM | 8.16 |
| CHEMBL6055997 | — | IC50 | = | 8.6 | nM | 8.07 |
| CHEMBL5810071 | — | IC50 | = | 10.2 | nM | 7.99 |
| CHEMBL5985547 | — | IC50 | = | 10.3 | nM | 7.99 |
| CHEMBL5962871 | — | IC50 | = | 10.6 | nM | 7.97 |
| CHEMBL5800735 | — | IC50 | = | 10.7 | nM | 7.97 |
| CHEMBL5775203 | — | IC50 | = | 11.4 | nM | 7.94 |
| CHEMBL6002147 | — | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL5991064 | — | IC50 | = | 12.8 | nM | 7.89 |
| CHEMBL5837159 | — | IC50 | = | 13.2 | nM | 7.88 |