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Assay Detail

CHEMBL5735701

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition In Vito Assay: The investigations on the inhibition of the recombinant TASK-1 and TASK-3 channels were carried out using stably transfected CHO cells. Here, the compounds according to the invention were tested with administration of 40 mM of potassium chloride in the presence of a voltage-sensitive dye for high-throughput screening of potassium channel from Molecular Devices FLIPR Application Note: Measuring membrane potential using the FLIPR membrane potential assay kit on Fluorometric Imaging Plate Reader (FLIPR) systems. The activity of the test substances was determined as their ability to inhibit a depolarization induced in the recombinant cells by 40 mM potassium chloride.
258
Total Activities
77
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Potassium channel subfamily K member 9 (CHEMBL2321614)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-phenyl-3-(piperazinomethyl)imidazo[1,2-A]pyridine derivatives as blockers of task-1 and task-2 channels, for the treatment of sleep-related breathing disorders
(2020)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 86 6.76 8.98
kon 86 - -
k_off 86 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6016320 6 8.98
CHEMBL5979320 6 8.64
CHEMBL5948731 3 8.52
CHEMBL6050684 3 8.28
CHEMBL5781241 6 8.21
CHEMBL5928540 3 8.04
CHEMBL5172358 3 8.03
CHEMBL5932576 3 8.00
CHEMBL5955132 3 7.96
CHEMBL5936147 3 7.89
CHEMBL5881187 3 7.85
CHEMBL5189300 6 7.82
CHEMBL5955974 3 7.80
CHEMBL5922163 3 7.77
CHEMBL5833962 3 7.77
CHEMBL5883778 6 7.70
CHEMBL5976530 6 7.55
CHEMBL6056886 3 7.52
CHEMBL5953514 6 7.42
CHEMBL5877257 3 7.28
CHEMBL5756587 3 7.22
CHEMBL5918386 3 7.20
CHEMBL5969271 3 7.20
CHEMBL6028256 3 7.15
CHEMBL5932536 6 7.10
CHEMBL5742220 3 7.01
CHEMBL5941398 3 7.00
CHEMBL5870683 3 6.96
CHEMBL6013481 3 6.92
CHEMBL6058665 3 6.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6016320 IC50 = 1.04 nM 8.98
CHEMBL5979320 IC50 = 2.3 nM 8.64
CHEMBL5948731 IC50 = 3.0 nM 8.52
CHEMBL6050684 IC50 = 5.2 nM 8.28
CHEMBL5781241 IC50 = 6.2 nM 8.21
CHEMBL5928540 IC50 = 9.1 nM 8.04
CHEMBL5172358 IC50 = 9.4 nM 8.03
CHEMBL5932576 IC50 = 10.0 nM 8.00
CHEMBL5955132 IC50 = 11.0 nM 7.96
CHEMBL5936147 IC50 = 13.0 nM 7.89
CHEMBL5881187 IC50 = 14.0 nM 7.85
CHEMBL5189300 IC50 = 15.0 nM 7.82
CHEMBL5955974 IC50 = 16.0 nM 7.80
CHEMBL6016320 IC50 = 17.0 nM 7.77
CHEMBL5922163 IC50 = 17.0 nM 7.77
CHEMBL5833962 IC50 = 17.0 nM 7.77
CHEMBL5979320 IC50 = 18.0 nM 7.75
CHEMBL5883778 IC50 = 20.0 nM 7.70
CHEMBL5883778 IC50 = 21.8 nM 7.66
CHEMBL5976530 IC50 = 27.9 nM 7.55
CHEMBL5976530 IC50 = 28.0 nM 7.55
CHEMBL6056886 IC50 = 30.0 nM 7.52
CHEMBL5953514 IC50 = 38.2 nM 7.42
CHEMBL5189300 IC50 = 46.0 nM 7.34
CHEMBL5877257 IC50 = 52.0 nM 7.28
CHEMBL5781241 IC50 = 56.0 nM 7.25
CHEMBL5756587 IC50 = 60.0 nM 7.22
CHEMBL5918386 IC50 = 63.0 nM 7.20
CHEMBL5969271 IC50 = 63.0 nM 7.20
CHEMBL6028256 IC50 = 71.0 nM 7.15
CHEMBL5932536 IC50 = 79.6 nM 7.10
CHEMBL5742220 IC50 = 98.0 nM 7.01
CHEMBL5941398 IC50 = 99.0 nM 7.00
CHEMBL5870683 IC50 = 110.0 nM 6.96
CHEMBL6058665 IC50 = 120.0 nM 6.92
CHEMBL6013481 IC50 = 120.0 nM 6.92
CHEMBL5995367 IC50 = 130.0 nM 6.89
CHEMBL5822460 IC50 = 140.0 nM 6.85
CHEMBL5932536 IC50 = 141.0 nM 6.85
CHEMBL5885901 IC50 = 150.0 nM 6.82
CHEMBL5763659 IC50 = 160.0 nM 6.80
CHEMBL5767153 IC50 = 190.0 nM 6.72
CHEMBL5855154 IC50 = 190.0 nM 6.72
CHEMBL5937118 IC50 = 200.0 nM 6.70
CHEMBL5953514 IC50 = 225.0 nM 6.65
CHEMBL5870080 IC50 = 240.0 nM 6.62
CHEMBL6061336 IC50 = 240.0 nM 6.62
CHEMBL6049147 IC50 = 300.0 nM 6.52
CHEMBL6001517 IC50 = 310.0 nM 6.51
CHEMBL6003079 IC50 = 350.0 nM 6.46