Assay Detail
Binding
CHEMBL5736187
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Biochemical Inhibition Assay: The biochemical inhibition of four PI3K isoforms by the Formula I compounds of Table 1. In addition, two clinically tested PI3K compounds, taselisib and pictilisib are included as comparators. The representative compounds of the invention exhibit strong activity against PI3Kα, and exhibit significantly enhanced selectivity relative to the other isoforms PI3Kβ, PI3Kδ, and PI3Kγ when compared to taselisib (GDC-0032) and pictilisib (GDC-0941). In particular, the selectivity ratios in the second from the right column of Table 2A show that each Formula I compounds 101-107 has a PI3K alpha to delta selectivity ratio far higher than taselisib or pictilisib. In fact, both taselisib and pictilisib have stronger activity against PI3K delta than against PI3K alpha, i.e. their selectivity ratios are less than 1. The selectivity ratios of Formula I compound 101-107 range from 301-fold to 634-fold.
66
Total Activities
21
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform (CHEMBL3267) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Benzoxazepin oxazolidinone compounds and methods of use
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 22 | 8.32 | 10.10 |
| kon | 22 | - | - |
| k_off | 22 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2387080 | TASELISIB | 3.0 | 6 | 10.10 |
| CHEMBL5847452 | — | — | 3 | 9.41 |
| CHEMBL3770993 | — | — | 3 | 9.38 |
| CHEMBL5784302 | — | — | 3 | 9.04 |
| CHEMBL6019298 | — | — | 3 | 8.98 |
| CHEMBL521851 | PICTILISIB | 2.0 | 3 | 8.81 |
| CHEMBL5823247 | — | — | 3 | 8.77 |
| CHEMBL5916419 | — | — | 3 | 8.71 |
| CHEMBL5171276 | — | — | 3 | 8.60 |
| CHEMBL3771364 | — | — | 3 | 8.16 |
| CHEMBL6055974 | — | — | 3 | 8.03 |
| CHEMBL5784355 | — | — | 3 | 7.95 |
| CHEMBL4650215 | INAVOLISIB | 4.0 | 3 | 7.91 |
| CHEMBL5916599 | — | — | 3 | 7.78 |
| CHEMBL5789652 | — | — | 3 | 7.76 |
| CHEMBL5875257 | — | — | 3 | 7.66 |
| CHEMBL5916259 | — | — | 3 | 7.62 |
| CHEMBL5186155 | — | — | 3 | 7.52 |
| CHEMBL5196718 | — | — | 3 | 7.42 |
| CHEMBL5967979 | — | — | 3 | 6.71 |
| CHEMBL5748747 | — | — | 3 | 6.54 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2387080 | TASELISIB | Ki | = | 0.079 | nM | 10.10 |
| CHEMBL2387080 | TASELISIB | Ki | = | 0.079 | nM | 10.10 |
| CHEMBL5847452 | — | Ki | = | 0.39 | nM | 9.41 |
| CHEMBL3770993 | — | Ki | = | 0.417 | nM | 9.38 |
| CHEMBL5784302 | — | Ki | = | 0.92 | nM | 9.04 |
| CHEMBL6019298 | — | Ki | = | 1.05 | nM | 8.98 |
| CHEMBL521851 | PICTILISIB | Ki | = | 1.54 | nM | 8.81 |
| CHEMBL5823247 | — | Ki | = | 1.7 | nM | 8.77 |
| CHEMBL5916419 | — | Ki | = | 1.96 | nM | 8.71 |
| CHEMBL5171276 | — | Ki | = | 2.5 | nM | 8.60 |
| CHEMBL3771364 | — | Ki | = | 6.94 | nM | 8.16 |
| CHEMBL6055974 | — | Ki | = | 9.41 | nM | 8.03 |
| CHEMBL5784355 | — | Ki | = | 11.3 | nM | 7.95 |
| CHEMBL4650215 | INAVOLISIB | Ki | = | 12.2 | nM | 7.91 |
| CHEMBL5916599 | — | Ki | = | 16.7 | nM | 7.78 |
| CHEMBL5789652 | — | Ki | = | 17.5 | nM | 7.76 |
| CHEMBL5875257 | — | Ki | = | 21.8 | nM | 7.66 |
| CHEMBL5916259 | — | Ki | = | 24.1 | nM | 7.62 |
| CHEMBL5186155 | — | Ki | = | 30.3 | nM | 7.52 |
| CHEMBL5196718 | — | Ki | = | 37.7 | nM | 7.42 |
| CHEMBL5967979 | — | Ki | = | 197.0 | nM | 6.71 |
| CHEMBL5748747 | — | Ki | = | 286.0 | nM | 6.54 |
| CHEMBL5784355 | — | k_off | = | - | s-1 | - |
| CHEMBL6055974 | — | kon | = | - | — | - |
| CHEMBL6055974 | — | k_off | = | - | s-1 | - |
| CHEMBL5823247 | — | kon | = | - | — | - |
| CHEMBL5186155 | — | kon | = | - | — | - |
| CHEMBL2387080 | TASELISIB | kon | = | - | — | - |
| CHEMBL2387080 | TASELISIB | k_off | = | - | s-1 | - |
| CHEMBL521851 | PICTILISIB | kon | = | - | — | - |
| CHEMBL521851 | PICTILISIB | k_off | = | - | s-1 | - |
| CHEMBL4650215 | INAVOLISIB | kon | = | - | — | - |
| CHEMBL4650215 | INAVOLISIB | k_off | = | - | s-1 | - |
| CHEMBL5748747 | — | kon | = | - | — | - |
| CHEMBL5748747 | — | k_off | = | - | s-1 | - |
| CHEMBL5196718 | — | kon | = | - | — | - |
| CHEMBL5784355 | — | kon | = | - | — | - |
| CHEMBL5916259 | — | k_off | = | - | s-1 | - |
| CHEMBL5823247 | — | k_off | = | - | s-1 | - |
| CHEMBL5916419 | — | kon | = | - | — | - |
| CHEMBL6019298 | — | k_off | = | - | s-1 | - |
| CHEMBL6019298 | — | kon | = | - | — | - |
| CHEMBL5784302 | — | k_off | = | - | s-1 | - |
| CHEMBL5916419 | — | k_off | = | - | s-1 | - |
| CHEMBL5875257 | — | k_off | = | - | s-1 | - |
| CHEMBL5875257 | — | kon | = | - | — | - |
| CHEMBL5171276 | — | kon | = | - | — | - |
| CHEMBL5916259 | — | kon | = | - | — | - |
| CHEMBL5784302 | — | kon | = | - | — | - |
| CHEMBL5171276 | — | k_off | = | - | s-1 | - |