Assay Detail
Binding
CHEMBL5737838
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition Assay: Pooled human liver microsome suspension (20 mg/mL) was diluted with phosphate buffer to obtain a 5 mg/mL suspension. A solution of NADPH was prepared in phosphate buffer at a concentration of 5 mM. Separate stock solutions of each substrate were prepared in DMSO:MeCN (50:50 v/v), mixed, and diluted in phosphate buffer to obtain a single solution containing each substrate at five times its experimentally determined Km concentration.
12
Total Activities
4
Compounds Tested
3
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Metalloenzyme inhibitor compounds
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 4.93 | 5.10 |
| kon | 4 | - | - |
| k_off | 4 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL106 | FLUCONAZOLE | 4.0 | 3 | 5.10 |
| CHEMBL638 | VORICONAZOLE | 4.0 | 3 | 4.89 |
| CHEMBL3311223 | — | — | 3 | 4.80 |
| CHEMBL3311224 | — | — | 3 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL106 | FLUCONAZOLE | IC50 | = | 8000.0 | nM | 5.10 |
| CHEMBL638 | VORICONAZOLE | IC50 | = | 13000.0 | nM | 4.89 |
| CHEMBL3311223 | — | IC50 | = | 16000.0 | nM | 4.80 |
| CHEMBL3311223 | — | kon | = | - | — | - |
| CHEMBL3311223 | — | k_off | = | - | s-1 | - |
| CHEMBL3311224 | — | IC50 | > | 60000.0 | nM | - |
| CHEMBL3311224 | — | kon | = | - | — | - |
| CHEMBL3311224 | — | k_off | = | - | s-1 | - |
| CHEMBL106 | FLUCONAZOLE | kon | = | - | — | - |
| CHEMBL106 | FLUCONAZOLE | k_off | = | - | s-1 | - |
| CHEMBL638 | VORICONAZOLE | kon | = | - | — | - |
| CHEMBL638 | VORICONAZOLE | k_off | = | - | s-1 | - |