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Assay Detail

CHEMBL619242

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of 5-lipoxygenase measured by the reduction of leukotriene B4 (LTB4) in intact basophilic rat leukemia cells
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL312)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Design of 5-(3,5-di-tert-butyl-4-hydroxyphenyl)-1,3,4-thiadiazoles, -1,3,4-oxadiazoles, and -1,2,4-triazoles as orally-active, nonulcerogenic antiinflammatory agents.
Mullican MD, Wilson MW, Connor DT, Kostlan CR, Schrier DJ, Dyer RD.
J Med Chem (1993) 36 : 1090 -1099
PubMed 8478906 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 5.65 6.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL12395 1 6.68
CHEMBL273941 1 6.24
CHEMBL12219 1 6.16
CHEMBL12809 1 5.96
CHEMBL12532 1 5.92
CHEMBL16977 1 5.85
CHEMBL417073 1 5.85
CHEMBL12572 1 5.80
CHEMBL94851 1 5.75
CHEMBL12302 1 5.62
CHEMBL275909 1 5.62
CHEMBL122193 1 5.55
CHEMBL12234 1 5.35
CHEMBL276916 1 5.32
CHEMBL12154 1 5.29
CHEMBL16827 1 5.23
CHEMBL12483 1 4.96
CHEMBL509 MECLOFENAMIC ACID 4.0 1 4.62
CHEMBL275445 1 -
CHEMBL273956 1 -
CHEMBL12371 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL12395 IC50 = 210.0 nM 6.68
CHEMBL273941 IC50 = 580.0 nM 6.24
CHEMBL12219 IC50 = 700.0 nM 6.16
CHEMBL12809 IC50 = 1100.0 nM 5.96
CHEMBL12532 IC50 = 1200.0 nM 5.92
CHEMBL16977 IC50 = 1400.0 nM 5.85
CHEMBL417073 IC50 = 1400.0 nM 5.85
CHEMBL12572 IC50 = 1600.0 nM 5.80
CHEMBL94851 IC50 = 1800.0 nM 5.75
CHEMBL12302 IC50 = 2400.0 nM 5.62
CHEMBL275909 IC50 = 2400.0 nM 5.62
CHEMBL122193 IC50 = 2800.0 nM 5.55
CHEMBL12234 IC50 = 4500.0 nM 5.35
CHEMBL276916 IC50 = 4800.0 nM 5.32
CHEMBL12154 IC50 = 5100.0 nM 5.29
CHEMBL16827 IC50 = 5900.0 nM 5.23
CHEMBL12483 IC50 = 11000.0 nM 4.96
CHEMBL509 MECLOFENAMIC ACID IC50 = 24000.0 nM 4.62
CHEMBL275445 IC50 > 10000.0 nM -
CHEMBL273956 IC50 > 32000.0 nM -
CHEMBL12371 IC50 > 32000.0 nM -