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Assay Detail

CHEMBL621853

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Functional
Inhibition of autophosphorylation of epidermal growth factor receptor in A431 cells in culture
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type A-431
Confidence 9 — Direct single protein target
Curated By Expert

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tyrosine kinase inhibitors. 14. Structure-activity relationships for methylamino-substituted derivatives of 4-[(3-bromophenyl)amino]-6-(methylamino)-pyrido[3,4-d]pyrimidine (PD 158780), a potent and specific inhibitor of the tyrosine kinase activity of receptors for the EGF family of growth factors.
Rewcastle GW, Murray DK, Elliott WL, Fry DW, Howard CT, Nelson JM, Roberts BJ, Vincent PW, Showalter HD, Winters RT, Denny WA.
J Med Chem (1998) 41 : 742 -751
PubMed 9513602 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 7.63 8.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL165574 1 8.23
CHEMBL162142 1 8.20
CHEMBL162622 1 8.10
CHEMBL162223 1 8.09
CHEMBL166307 1 8.05
CHEMBL162981 1 8.04
CHEMBL53753 1 7.89
CHEMBL165508 1 7.89
CHEMBL51853 1 7.80
CHEMBL166358 1 7.80
CHEMBL349070 1 7.72
CHEMBL165731 1 7.70
CHEMBL53711 1 7.68
CHEMBL162821 1 7.64
CHEMBL166255 1 7.57
CHEMBL433520 1 7.55
CHEMBL162034 1 7.37
CHEMBL162053 1 7.34
CHEMBL165630 1 7.15
CHEMBL162719 1 6.99
CHEMBL163369 1 6.64
CHEMBL163168 1 6.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL165574 IC50 = 5.9 nM 8.23
CHEMBL162142 IC50 = 6.3 nM 8.20
CHEMBL162622 IC50 = 7.9 nM 8.10
CHEMBL162223 IC50 = 8.1 nM 8.09
CHEMBL166307 IC50 = 8.9 nM 8.05
CHEMBL162981 IC50 = 9.1 nM 8.04
CHEMBL53753 IC50 = 13.0 nM 7.89
CHEMBL165508 IC50 = 13.0 nM 7.89
CHEMBL51853 IC50 = 16.0 nM 7.80
CHEMBL166358 IC50 = 16.0 nM 7.80
CHEMBL349070 IC50 = 19.0 nM 7.72
CHEMBL165731 IC50 = 20.0 nM 7.70
CHEMBL53711 IC50 = 21.0 nM 7.68
CHEMBL162821 IC50 = 23.0 nM 7.64
CHEMBL166255 IC50 = 27.0 nM 7.57
CHEMBL433520 IC50 = 28.0 nM 7.55
CHEMBL162034 IC50 = 43.0 nM 7.37
CHEMBL162053 IC50 = 46.0 nM 7.34
CHEMBL165630 IC50 = 71.0 nM 7.15
CHEMBL162719 IC50 = 102.0 nM 6.99
CHEMBL163369 IC50 = 230.0 nM 6.64
CHEMBL163168 IC50 = 449.0 nM 6.35