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Assay Detail

CHEMBL643956

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding activity against alpha-2 adrenergic receptor from calf cerebral cortex, using [3H]clonidine as the radioligand
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Cerebral cortex
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Alpha-2A adrenergic receptor (CHEMBL4744)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Pyridinylpiperazines, a new class of selective alpha 2-adrenoceptor antagonists.
Saari WS, Halczenko W, King SW, Huff JR, Guare JP, Hunt CA, Randall WC, Anderson PS, Lotti VJ, Taylor DA.
J Med Chem (1983) 26 : 1696 -1701
PubMed 6139479 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 7.42 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL10316 IDAZOXAN 3.0 1 8.82
CHEMBL47415 1 8.57
CHEMBL50017 1 8.54
CHEMBL280514 1 8.30
CHEMBL298004 1 8.10
CHEMBL48833 1 8.09
CHEMBL295921 1 7.96
CHEMBL48137 1 7.85
CHEMBL6437 MIANSERIN 4.0 1 7.77
CHEMBL45773 1 7.75
CHEMBL10347 RAUWOLSCINE 1 7.75
CHEMBL9841 1 7.60
CHEMBL296653 1 7.58
CHEMBL18094 1 7.43
CHEMBL295764 1 7.38
CHEMBL15245 YOHIMBINE 3.0 1 7.31
CHEMBL299007 1 7.13
CHEMBL45663 1 7.01
CHEMBL47471 1 6.92
CHEMBL47740 1 6.41
CHEMBL45665 1 6.36
CHEMBL50016 1 6.30
CHEMBL296787 1 5.87
CHEMBL47688 1 5.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL10316 IDAZOXAN Ki = 1.5 nM 8.82
CHEMBL47415 Ki = 2.7 nM 8.57
CHEMBL50017 Ki = 2.9 nM 8.54
CHEMBL280514 Ki = 5.0 nM 8.30
CHEMBL298004 Ki = 7.9 nM 8.10
CHEMBL48833 Ki = 8.2 nM 8.09
CHEMBL295921 Ki = 11.0 nM 7.96
CHEMBL48137 Ki = 14.0 nM 7.85
CHEMBL6437 MIANSERIN Ki = 17.0 nM 7.77
CHEMBL45773 Ki = 18.0 nM 7.75
CHEMBL10347 RAUWOLSCINE Ki = 18.0 nM 7.75
CHEMBL9841 Ki = 25.0 nM 7.60
CHEMBL296653 Ki = 26.0 nM 7.58
CHEMBL18094 Ki = 37.0 nM 7.43
CHEMBL295764 Ki = 42.0 nM 7.38
CHEMBL15245 YOHIMBINE Ki = 49.0 nM 7.31
CHEMBL299007 Ki = 74.0 nM 7.13
CHEMBL45663 Ki = 97.0 nM 7.01
CHEMBL47471 Ki = 120.0 nM 6.92
CHEMBL47740 Ki = 390.0 nM 6.41
CHEMBL45665 Ki = 440.0 nM 6.36
CHEMBL50016 Ki = 500.0 nM 6.30
CHEMBL296787 Ki = 1340.0 nM 5.87
CHEMBL47688 Ki = 5200.0 nM 5.28